Nocturnal aircraft noise disturbs sleep and impairs recuperation. We investigated in laboratory and field studies whether noise-induced sleep fragmentation is associated with performance impairments in a psychomotor vigilance task (PVT) and a memory search task. In the laboratory, 112 participants were exposed to aircraft noise during 9 consecutive nights. In the field, 64 participants were examined during 9 consecutive nights in the vicinity of Cologne/Bonn airport. Reaction time, signal detection performance and subjective task load were recorded. Dose-response relationships showed significant, linear impairments in reaction times. In the laboratory, reaction time in PVT increased with 0.13 ms/dB equivalent noise level (LAeq) plus 0.02 ms/noise event. In the field study, reaction time increased with 0.3 ms/dB LAeq. Participants worked significantly less accurate after nocturnal noise exposure. Influences of LAeq and number of noise events on daytime performance were small but consistent and significant, stressing the potential public health impact of nocturnal noise exposure.