Identification and disruption of a rhoptry-localized homologue of sodium hydrogen exchangers in Toxoplasma gondii

被引：28
作者：
Karasov, AO
Boothroyd, JC
Arrizabalaga, G [1 ]
机构：
[1] Univ Idaho, Dept Microbiol Mol Biol & Biochem, Moscow, ID 83844 USA
[2] Stanford Univ, Dept Microbiol & Immunol, Sch Med, Stanford, CA 94305 USA
来源：
INTERNATIONAL JOURNAL FOR PARASITOLOGY | 2005年 / 35卷 / 03期
关键词：
toxoplasma; sodium hydrogen exchanger; rhoptry; osmoregulation;
D O I：
10.1016/j.ijpara.2004.11.015
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Na+/H+ exchangers (NHEs) are ubiquitous membrane proteins that catalyze the exchange of Na+ for H+ and are critical in pH and cell volume regulation, as well as osmotolerance. In this study, we identify and characterize a novel NHE, TgNHE2, in Toxoplasma gondii. Immunofluorescence studies show that TgNHE2 is localized to the rhoptries, secretory organelles involved in invasion. TgNHE2 is the first intracellular NHE to be characterized in a protozoan parasite and its localization suggests possible roles for the rhoptries in osmotolerance and/or as secretory lysosomes-like granules. (c) 2004 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码：285 / 291
页数：7
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