Potential of basidiomycetes Marasmiellus sp. and Ganoderma lucidum in xylanase enzyme production and its activity using agroindustry waste

Nowadays, agro-industrial waste such as kemiri sunan (Reutealis trisperma (Blanco) Airy Shaw) shell is abundant along with the development of biodiesel production made from kemiri sunan. The production of xylanase from kemiri sunan waste was carried out by solid state fermentation method using Basidiomycetes Marasmiellus sp and Ganoderma lucidum. Enzyme activity of xylanase was tested by measuring the amount of reducing sugar liberated from the medium by reactants using DNS method. The highest of enzyme activity resulted by fermentation using 4% of substrate concentration either by Marasmiellus sp or G. lucidum. Fermentation by Marasmiellus sp produced the highest xylanase enzyme activity amounted for 190.5 U ml(-1) within 48 hours. Meanwhile, the highest enzyme activity of xylanase fermentation by G. lucidum was at the value of 121.9 U ml(-1) within 72 hours and it still showed a tendency to increase.