Application of 3D Traction Force Microscopy to Mechanotransduction of Cell Clusters

被引:1
|
作者
Notbohm, Jacob [1 ]
Kim, Jin-Hong [2 ]
Asthagiri, Anand [3 ]
Ravichandran, Guruswami [1 ]
机构
[1] CALTECH, Div Engn & Appl Sci, 1200 E Calif Blvd,MC 105-50, Pasadena, CA 91125 USA
[2] Gwangju Inst Sci & Technol, Cell Dynam Res Ctr, Gwangju, South Korea
[3] Northeastern Univ, Dept Chem Engn, Boston, MA USA
基金
美国国家科学基金会;
关键词
3D traction force microscopy; mechanotransduction; digital volume correlation; LOCOMOTION; STRESSES;
D O I
10.4028/www.scientific.net/AMM.70.21
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
With increasing understanding of the important role mechanics plays in cell behavior, the experimental technique of traction force microscopy has grown in popularity over the past decade. While researchers have assumed that cells on a flat substrate apply tractions in only two dimensions, a finite element simulation is discussed here that demonstrates how cells apply tractions in all three dimensions. Three dimensional traction force microscopy is then used to experimentally confirm the finite element results. Finally, the implications that the traction distributions of cell clusters have on the study of inhibition of proliferation due to cell contact and scattering of cells in a cluster are discussed.
引用
收藏
页码:21 / +
页数:2
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