Protein-Protein Communication and Enzyme Activation Mediated by a Synthetic Chemical Transducer

被引：40

作者：

Peri-Naor, Ronny ^{[1
]}

Ilani, Tal ^{[2
]}

Motiei, Leila ^{[1
]}

Margulies, David ^{[1
]}

机构：

[1] Weizmann Inst Sci, Dept Organ Chem, IL-76100 Rehovot, Israel

[2] Weizmann Inst Sci, Dept Biol Struct, IL-76100 Rehovot, Israel

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2015年 / 137卷 / 30期

基金：

欧洲研究理事会;

关键词：

DNA; LOGIC; BINDING; MOLECULES; AFFINITY; CIRCUIT; PRODRUG; SENSE; CELL;

D O I：

10.1021/jacs.5b01123

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The design and function of A synthetic "chemical transducer" that can generate an unnatural communication channel between two proteins is described. Specifically, we show how this transducer enables platelet-derived growth factor to trigger (in vitro) the catalytic activity of glutathione-s-transferase (GST), which is not Its natural enzyme partner. GST activity can be further controlled by adding specific oligonucleotides that switch the enzymatic reaction: on and off: We also demonstrate that a molecular machine, which can regulate the function of an enzyme, could be used to change the way a prodrug is activated in a "programmable" manner.

引用

页码：9507 / 9510

页数：4

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