Quantitative analysis of senecavirus A in tissue samples from naturally infected newborn piglets

被引：18
作者：
Dall Agnol, Alais Maria [1 ,3 ]
Miyabe, Flavia Megumi [1 ,3 ]
Leme, Raquel Arruda [1 ,3 ]
Silva Oliveira, Thalita Evani [2 ]
Headley, Selwyn Arlington [2 ]
Alfieri, Amauri Alcindo [1 ,3 ]
Alfieri, Alice Fernandes [1 ,3 ]
机构：
[1] Univ Estadual Londrina, Dept Vet Prevent Med, Lab Anim Virol, Rodovia Celso Garcia Cid,Campus Univ,POB 10011, BR-86057970 Londrina, Parana, Brazil
[2] Univ Estadual Londrina, Dept Vet Prevent Med, Lab Anim Pathol, Rodovia Celso Garcia Cid,Campus Univ,POB 10011, BR-86057970 Londrina, Parana, Brazil
[3] Univ Estadual Londrina, Dept Vet Prevent Med, Multiuser Anim Hlth Lab, Mol Biol Unit, Rodovia Celso Garcia Cid,Campus Univ,POB 10011, BR-86057970 Londrina, Parana, Brazil
来源：
ARCHIVES OF VIROLOGY | 2018年 / 163卷 / 02期
关键词：
Swine; SVA; Multisystemic infection; qRTPCR; Viral load; VESICULAR DISEASE; NEONATAL-MORTALITY; PIGS; LESIONS;
D O I：
10.1007/s00705-017-3630-8
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
In this study, we determined the distribution of senecavirus A (SVA) and viral RNA load in different organs and tissues of naturally infected piglets. A TaqMan-based qRT-PCR assay was performed using RNA extracted from brainstem, cerebellum, cerebrum, heart, kidney, liver, lungs, small intestine, spleen, urinary bladder, and tonsils of seven newborn piglets. SVA was detected in 57 out of 70 tissue samples (81.4%). Viral loads ranged from 4.07 to 10.38 log(10) genomic copies per g of tissue. The results show that SVA has tropism for various organs in naturally infected newborn piglets, especially for tonsils, spleen, lungs, and liver. Lymphoid organs had the highest viral loads and may be important sites for SVA replication.
引用
收藏
页码：527 / 531
页数：5
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