Crocinitomix algicola sp nov., isolated from Gracilaria blodgettii

A Gram-stain-negative, strictly aerobic, non-flagellated, yellow-pigmented, rod-shaped bacterial strain, designated 0182(T), was isolated from Gracilaria blodgettii, an algae of phylum Rhodophyta collected from coast of Lingshui county, Hainan, China (110 degrees 03' 44.2" E, 18 degrees 24' 29.8" N). The strain grew optimally at 30 degrees C, pH 7.0-7.5 and in the presence of 2.0-3.0% (w/v) NaCl. Cells of strain 0182(T) were approximately 0.9-2.5 mu m in length and 0.2-0.4 mu m in width. The major respiratory quinone was MK-7. The predominant cellular fatty acids were iso-C-15 : 0, C-15 : 0 and iso-C-17 : 0 3-OH. The major polar lipids were phosphoaminolipid, glycolipid, two unknown aminolipids and four unknown lipids. The DNA G+C content was approximately 35.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 0182(T) was phylogenetically related to members of the genus Crocinitomix and was closely related to Crocinitomix catalasitica with 94.6% sequence similarity. On the basis of genotypic and phenotypic characteristics and phylogenetic evidence, strain 0182(T) is thought to represent a novel species of the genus Crocinitomix, for which the name Crocinitomix algicola sp. nov. is proposed. The type strain is 0182(T) (=KCTC 42868(T)=MCCC 1H00128(T)).