Complete genome characterization of porcine circovirus 3 recovered from wild boars in Southern Brazil

被引：11

作者：

Varela, Ana Paula Muterle ^{[1
]}

Loiko, Marcia Regina ^{[1
]}

Andrade, Juliana da Silva ^{[2
]}

Tochetto, Caroline ^{[1
]}

Cibulski, Samuel Paulo ^{[1
]}

Lima, Diane Alves ^{[1
]}

Weber, Matheus Nunes ^{[1
]}

Roehe, Paulo Michel ^{[1
]}

Mayer, Fabiana Quoos ^{[2
]}

机构：

[1] Univ Fed Rio Grande do Sul, Inst Ciencias Basicas Saude ICBS, Dept Microbiol Imunol & Parasitol, Lab Virol, Porto Alegre, RS, Brazil

[2] Secretaria Agr Pecuaria & Desenvolvimento Rural, Ctr Pesquisa Saude Anim, Inst Pesquisas Vet Desiderio Finamor, Dept Diagnost & Pesquisa Agr, Eldorado Do Sul, Brazil

来源：

TRANSBOUNDARY AND EMERGING DISEASES | 2021年 / 68卷 / 02期

关键词：

circovirus; high-throughput sequencing; PCR; wild boars; GENETIC-CHARACTERIZATION; MOSAIC STRUCTURE; SEQUENCE; TYPE-3; RECOMBINATION; TUBERCULOSIS; INFECTION; DISEASE; DNA;

D O I：

10.1111/tbed.13679

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

In the present study, the complete nucleotide sequence of porcine circovirus 3 (PCV3) recovered from wild boars lymph nodes is described. The full genome was named PCV3-wb/Br/RS and comprises 2,000 nucleotides with two open reading frames (ORFs) with a stem-loop motif in intergenic region. The ORFs are oriented in opposite directions and encode the putative capsid (Cap) and replicase (Rep) proteins. Based on amino acid motif analysis, PCV3-wb/Br/RS as well as most of the sequences from wild boars are classified as PCV3b. Phylogenetic analysis including 97 PCV3 sequences available in databases showed that the PCV3-wb/Br/RS genome is more closely related to genomes recovered in Spain, China, Germany and Denmark. Phylogenetic inferences among PCV3-wb/Br/RS and other circoviruses confirmed that these seem to have a most recent common ancestor with bat-associated circoviruses. In addition, PCV3 infection was investigated by real-time PCR in a cohort of 80 wild boars in Southern Brazil. A total of 29 animals (36.3%) were PCV3-positive leading the conclusion that PCV3 is circulating in the wild boar population in Southern Brazil. The role played by PCV3-like infections in wild boars and the risk these could pose to commercial swine production within that region remains to be further investigated.

引用

页码：240 / 247

页数：8

共 53 条

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