HPLC-PDA-MS/MS Characterization of Bioactive Secondary Metabolites from Turraea fischeri Bark Extract and Its Antioxidant and Hepatoprotective Activities In Vivo

被引：20

作者：

Sobeh, Mansour ^{[1
]}

Mahmoud, Mona F. ^{[2
]}

Sabry, Omar M. ^{[3
]}

Adel, Rasha ^{[4
]}

Dmirieh, Malak ^{[1
]}

El-Shazly, Assem M. ^{[4
]}

Wink, Michael ^{[1
]}

机构：

[1] Heidelberg Univ, Inst Pharm & Mol Biotechnol, Neuenheimer Feld 364, D-69120 Heidelberg, Germany

[2] Zagazig Univ, Dept Pharmacol & Toxicol, Fac Pharm, Zagazig 44519, Egypt

[3] Cairo Univ, Dept Pharmacognosy, Coll Pharm, Cairo 11562, Egypt

[4] Zagazig Univ, Dept Pharmacognosy, Fac Pharm, Zagazig 44519, Egypt

来源：

MOLECULES | 2017年 / 22卷 / 12期

关键词：

Turraea fischeri; flavonolignan; cinchonains; HPLC-PDA-ESI-MS; MS; antioxidant; hepatoprotection; CAENORHABDITIS-ELEGANS; RICH EXTRACT; LIFE-SPAN; PLANTS; IDENTIFICATION; CONSTITUENTS; RESISTANCE;

D O I：

10.3390/molecules22122089

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Turraea fischeri is an East African traditional herb, which is widely used in traditional medicine. In this study, we profiled the secondary metabolites in the methanol extract of T. fischeri bark using HPLC-PDA-ESI-MS/MS, and 20 compounds were tentatively identified. Several isomers of the flavonolignan cinchonain-I and bis-dihydroxyphenylpropanoid-substituted catechin hexosides dominated the extract. Robust in vitro and in vivo antioxidant properties were observed in 1,1-diphenyl-2-picrylhydrazyl radical scavenging assay (DPPH) and ferric reducing antioxidant power (FRAP) assay, and in the model organism Caenorhabditis elegans. Additionally, the extract exhibited promising hepatoprotective activities in D-galactosamine (D-GaIN) treated rats. A significant reduction in the elevated levels of aspartate aminotransferase (AST), total bilirubin, gamma-glutamyltransferase (GGT), and malondialdehyde (MDA) and increase of glutathione (GSH) was observed in rats treated with the bark extract in addition to D-galactosamine when compared with rats treated with D-galactosamine alone. In conclusion, T. fischeri is apromising candidate for health-promoting and for pharmaceutical applications.

引用

页数：14

共 28 条

[1] Epigallocatechin gallate inhibits beta amyloid oligomerization in Caenorhabditis elegans and affects the daf-2/insulin-like signaling pathway [J].