Long-Lived Phosphorescent Iridium(III) Complexes Conjugated with Cationic Polyfluorenes for Heparin Sensing and Cellular Imaging

被引：27

作者：

Jiang, Jiayang ^{[1
,2
]}

Zhang, Chuanqi ^{[1
,2
]}

Lin, Wenpeng ^{[1
,2
]}

Liu, Yahong ^{[1
,2
]}

Liu, Shujuan ^{[1
,2
]}

Xu, Yunjian ^{[1
,2
]}

Zhao, Qiang ^{[1
,2
]}

Huang, Wei ^{[1
,2
,3
,4
]}

机构：

[1] Nanjing Univ Posts & Telecommun, Key Lab Organ Elect & Informat Displays, Nanjing 210023, Jiangsu, Peoples R China

[2] Nanjing Univ Posts & Telecommun, Inst Adv Mat IAM, Jiangsu Natl Synergist Innovat Ctr Adv Mat SICAM, Nanjing 210023, Jiangsu, Peoples R China

[3] Nanjing Tech Univ NanjingTech, Key Lab Flexible Elect KLOFE, Nanjing 211816, Jiangsu, Peoples R China

[4] Nanjing Tech Univ NanjingTech, Jiangsu Natl Synergist Innovat Ctr Adv Mat SICAM, Inst Adv Mat IAM, Nanjing 211816, Jiangsu, Peoples R China

来源：

MACROMOLECULAR RAPID COMMUNICATIONS | 2015年 / 36卷 / 07期

基金：

中国国家自然科学基金;

关键词：

biosensors; conjugated polyelectrolyte; heparin; Ir(III) complex; time-resolved photoluminescence technique; FLUORESCENCE LIFETIME; POLYMERS; PROBES; POLYELECTROLYTES; AGGREGATION; BIOSENSOR; CYSTEINE; DESIGN;

D O I：

10.1002/marc.201400654

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

The applications of conjugated polyelectrolytes in biosensing and bioimaging have attracted more and more research interests due to their excellent photophysical properties. In this work, a new series of conjugated polyelectrolytes containing long-lived phosphorescent Ir(III) complexes is designed and synthesized, which can be used for ratiometric and lifetime-based sensing of heparin utilizing the electrostatic interaction between cationic polymers and anionic heparin. By changing the ligand structures of Ir(III) complexes, the sensing performances of phosphorescent-conjugated polyelectrolytes (PCPEs) are optimized. In addition, the application of PCPEs in cellular imaging is carried out. These polymers can be applied for specific staining of cell membrane. Importantly, utilizing the long emission lifetime of phosphorescent signal of Ir(III) complexes, time-gated luminescent imaging is carried out, which can eliminate the short-lived background fluorescence interferences from the environment or biological samples.

引用

页码：640 / 646

页数：7

共 27 条

[1] Elemental tomography of cancer-cell spheroids reveals incomplete uptake of both Platinum(II) and Platinum(IV) complexes [J].