The heterogeneous motility of the Lyme disease spirochete in gelatin mimics dissemination through tissue

被引:78
作者
Harman, Michael W. [1 ,2 ,3 ,4 ,5 ,6 ]
Dunham-Ems, Star M. [3 ,4 ,5 ,6 ]
Caimano, Melissa J. [3 ,4 ,5 ,6 ]
Belperron, Alexia A. [7 ]
Bockenstedt, Linda K. [7 ]
Fu, Henry C. [8 ]
Radolf, Justin D. [3 ,4 ,5 ,6 ]
Wolgemuth, Charles W. [1 ,2 ]
机构
[1] Univ Connecticut, Ctr Hlth, Dept Cell Biol, Farmington, CT 06030 USA
[2] Univ Connecticut, Ctr Hlth, Ctr Cell Anal & Modeling, Farmington, CT 06030 USA
[3] Univ Connecticut, Ctr Hlth, Dept Med, Farmington, CT 06030 USA
[4] Univ Connecticut, Ctr Hlth, Dept Pediat, Farmington, CT 06030 USA
[5] Univ Connecticut, Ctr Hlth, Dept Genet & Dev Biol, Farmington, CT 06030 USA
[6] Univ Connecticut, Ctr Hlth, Dept Immunol, Farmington, CT 06030 USA
[7] Yale Univ, Dept Internal Med, New Haven, CT 06520 USA
[8] Univ Nevada, Dept Mech Engn, Reno, NV 89509 USA
基金
美国国家卫生研究院;
关键词
BORRELIA-BURGDORFERI; TICKS;
D O I
10.1073/pnas.1114362109
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The Lyme disease spirochete Borrelia burgdorferi exists in nature in an enzootic cycle that involves the arthropod vector Ixodes scapularis and mammalian reservoirs. To disseminate within and between these hosts, spirochetes must migrate through complex, polymeric environments such as the basement membrane of the tick midgut and the dermis of the mammal. To date, most research on the motility of B. burgdorferi has been done in media that do not resemble the tissue milieus that B. burgdorferi encounter in vivo. Here we show that the motility of Borrelia in gelatin matrices in vitro resembles the pathogen's movements in the chronically infected mouse dermis imaged by intravital microscopy. More specifically, B. burgdorferi motility in mouse dermis and gelatin is heterogeneous, with the bacteria transitioning between at least three different motility states that depend on transient adhesions to the matrix. We also show that B. burgdorferi is able to penetrate matrices with pore sizes much smaller than the diameter of the bacterium. We find a complex relationship between the swimming behavior of B. burgdorferi and the rheological properties of the gelatin, which cannot be accounted for by recent theoretical predictions for microorganism swimming in gels. Our results also emphasize the importance of considering borrelial adhesion as a dynamic rather than a static process.
引用
收藏
页码:3059 / 3064
页数:6
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