A novel steric effect-regulated isothermal exponential amplification technology for the one-step homogeneous sensing of proteins

被引：3

作者：

Wu, Wanghua ^{[1
]}

Pan, Wufan ^{[2
]}

Yu, Dongdong ^{[3
]}

Yuan, Zhen ^{[1
]}

Qin, Yazhou ^{[2
]}

Lu, Yuxiang ^{[2
]}

Zhang, Tao ^{[1
]}

Zhou, Jianguang ^{[1
]}

机构：

[1] Zhejiang Univ, State Key Lab Ind Control Technol, Inst Cyber Syst & Control, Res Ctr Analyt Instrumentat, Hangzhou 310027, Zhejiang, Peoples R China

[2] Zhejiang Univ, Dept Chem, Hangzhou 310027, Zhejiang, Peoples R China

[3] Zhejiang Univ, Hosp Zhejiang Univ, Hangzhou 310027, Zhejiang, Peoples R China

来源：

ANALYST | 2018年 / 143卷 / 04期

基金：

中国国家自然科学基金;

关键词：

MOLECULE-LINKED DNA; TERMINAL PROTECTION; ASSAY; BIOSENSOR; NANOCOMPOSITE; MATRICES; PLATFORM; BINDING; PROBES;

D O I：

10.1039/c7an01963g

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple and homogeneous technology, the steric effect-regulated isothermal exponential amplification reaction (SER-EXPAR), was developed to sense proteins. By using a small molecule linked DNA nanostructure, termed enzyme-binding hairpin (EBH), the protein-small molecule binding events could be readily sensed by utilizing the steric effect generated between the protein and enzyme. It set free the enzyme to be active again, thus regulating the amplification rate of EXPAR.

引用

收藏

页码：829 / 832

页数：4

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