Imaging cellular ultrastructures using expansion microscopy (U-ExM)

被引：300

作者：

Gambarotto, Davide ^{[1
]}

Zwettler, Fabian U. ^{[2
]}

Le Guennec, Maeva ^{[1
]}

Schmidt-Cernohorska, Marketa ^{[1
,7
]}

Fortun, Denis ^{[3
,8
]}

Borgers, Susanne ^{[1
]}

Heine, Joern ^{[4
]}

Schloetel, Jan-Gero ^{[4
]}

Reuss, Matthias ^{[4
]}

Unser, Michael ^{[5
]}

Boyden, Edward S. ^{[6
]}

Sauer, Markus ^{[2
]}

Hamel, Virginie ^{[1
]}

Guichard, Paul ^{[1
]}

机构：

[1] Univ Geneva, Dept Cell Biol, Sci 3, Geneva, Switzerland

[2] Univ Wurzburg, Dept Biotechnol & Biophys, Bioctr, Wurzburg, Germany

[3] Ecole Polytech Fed Lausanne, Signal Proc Core Ctr Biomed Imaging CIBM SP, Lausanne, Switzerland

[4] Abberior Instruments GmbH, Gottingen, Germany

[5] Ecole Polytech Fed Lausanne, Biomed Imaging Grp, Lausanne, Switzerland

[6] MIT, 77 Massachusetts Ave, Cambridge, MA 02139 USA

[7] Acad Sci Czech Republ, Inst Mol Genet, Lab Adaptat Immun, Prague, Czech Republic

[8] Univ Strasbourg, ICube, CNRS, Illkirch Graffenstaden, France

来源：

NATURE METHODS | 2019年 / 16卷 / 01期

基金：

瑞士国家科学基金会; 欧洲研究理事会;

关键词：

POLYGLUTAMYLATED TUBULIN; LOCALIZATION; PROTEINS; REVEALS;

D O I：

10.1038/s41592-018-0238-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Determining the structure and composition of macromolecular assemblies is a major challenge in biology. Here we describe ultrastructure expansion microscopy (U-ExM), an extension of expansion microscopy that allows the visualization of preserved ultrastructures by optical microscopy. This method allows for near-native expansion of diverse structures in vitro and in cells; when combined with super-resolution microscopy, it unveiled details of ultrastructural organization, such as centriolar chirality, that could otherwise be observed only by electron microscopy.

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收藏

页码：71 / +

页数：8

相关论文

共 30 条

[1] HyVolution-the smart path to confocal super-resolution [J].

Borlinghaus, Rolf Theodor ;

Kappel, Constantin .

NATURE METHODS, 2016, 13 (03) :I-III

[2] Expansion microscopy [J].

Chen, Fei ;

Tillberg, Paul W. ;

Boyden, Edward S. .

SCIENCE, 2015, 347 (6221) :543-548

[3]

Chozinski TJ, 2016, NAT METHODS, V13, P485, DOI [10.1038/NMETH.3833, 10.1038/nmeth.3833]

[4] Configurations of the Re-scan Confocal Microscope (RCM) for biomedical applications [J].

De Luca, G. M. R. ;

Desclos, E. ;

Breedijk, R. M. P. ;

Dolz-Edo, L. ;

Smits, G. J. ;

Nahidiazar, L. ;

Bielefeld, P. ;

Picavet, L. ;

Fitzsimons, C. P. ;

Hoebe, R. ;

Manders, E. M. M. .

JOURNAL OF MICROSCOPY, 2017, 266 (02) :166-177

[5] Reconstruction From Multiple Particles for 3D Isotropic Resolution in Fluorescence Microscopy [J].

Fortun, Denis ;

Guichard, Paul ;

Hamel, Virginie ;

Sorzano, Carlos Oscar S. ;

Banterle, Niccolo ;

Gonczy, Pierre ;

Unser, Michael .

IEEE TRANSACTIONS ON MEDICAL IMAGING, 2018, 37 (05) :1235-1246

[6] Expansion Stimulated Emission Depletion Microscopy (ExSTED) [J].

Gao, Mengfei ;

Maraspini, Riccardo ;

Beutel, Oliver ;

Zehtabian, Amin ;

Eickholt, Britta ;

Honigmann, Alf ;

Ewers, Helge .

ACS NANO, 2018, 12 (05) :4178-4185

[7] Strong signal increase in STED fluorescence microscopy by imaging regions of subdiffraction extent [J].

Goettfert, Fabian ;

Pleiner, Tino ;

Heine, Joern ;

Westphal, Volker ;

Goerlich, Dirk ;

Sahl, Steffen J. ;

Hell, Stefan W. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2017, 114 (09) :2125-2130

[8] Hybrid Structured Illumination Expansion Microscopy Reveals Microbial Cytoskeleton Organization [J].

Halpern, Aaron R. ;

Alas, Germain C. M. ;

Chozinski, Tyler J. ;

Paredez, Alexander R. ;

Vaughan, Joshua C. .

ACS NANO, 2017, 11 (12) :12677-12686

[9] Identification of Chlamydomonas Central Core Centriolar Proteins Reveals a Role for Human WDR90 in Ciliogenesis [J].

Hamel, Virginie ;

Steib, Emmanuelle ;

Hamelin, Romain ;

Armand, Florence ;

Borgers, Susanne ;

Fluckiger, Isabelle ;

Busso, Coralie ;

Olieric, Natacha ;

Sorzano, Carlos Oscar S. ;

Steinmetz, Michel O. ;

Guichard, Paul ;

Gonczy, Pierre .

CURRENT BIOLOGY, 2017, 27 (16) :2486-+

[10] Subdiffraction-resolution fluorescence imaging with conventional fluorescent probes [J].

Heilemann, Mike ;

van de Linde, Sebastian ;

Schuttpelz, Mark ;

Kasper, Robert ;

Seefeldt, Britta ;

Mukherjee, Anindita ;

Tinnefeld, Philip ;

Sauer, Markus .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2008, 47 (33) :6172-6176