Poly(lactic-co-glycolic acid) nanosphere as a vehicle for gene delivery to human cord blood-derived mesenchymal stem cells: comparison with polyethylenimine

被引：25

作者：

Gwak, So-Jung ^{[2
]}

Kim, Byung-Soo ^{[1
]}

机构：

[1] Hanyang Univ, Dept Bioengn, Seoul 133791, South Korea

[2] Hanyang Univ, Dept Chem Engn, Seoul 133791, South Korea

来源：

BIOTECHNOLOGY LETTERS | 2008年 / 30卷 / 07期

关键词：

gene delivery; non-viral vector; polyethylenimine; poly(lactic-co-glycolic acid) nanosphere;

D O I：

10.1007/s10529-008-9676-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Polyethylenimine (PEI) is one of the most extensively studied non-viral vectors but its cytotoxicity limits its clinical value. PLGA nanospheres are biocompatible and can facilitate sustained release of plasmid DNA. This study compares the cytotoxicity and long-term transgene expression between PLGA nanosphere and PEI. PLGA nanospheres were significantly less cytotoxic than PEI at various concentrations. PLGA nanospheres induced significantly higher transgene expression in vitro for a longer duration (21 days) than PEI. We conclude that PLGA nanospheres have potential as gene delivery vehicles for use in gene therapy for diseases in which a long-term therapeutic gene expression regimen is necessary.

引用

页码：1177 / 1182

页数：6

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