Raman tracking the activity of urease in saliva for healthcare

被引：29

作者：

Hu, Sen ^{[1
]}

Gao, Yun ^{[1
]}

Wu, Yiping ^{[1
]}

Guo, Xiaoyu ^{[1
]}

Ying, Ye ^{[1
]}

Wen, Ying ^{[1
]}

Yang, Haifeng ^{[1
]}

机构：

[1] Shanghai Normal Univ, Shanghai Key Lab Rare Earth Funct Mat, Shanghai Municipal Educ Comm Key Lab Mol Imaging, Educ Minist,Key Lab Resource Chem,Coll Chem & Mat, Shanghai 200234, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2019年 / 129卷

基金：

中国国家自然科学基金;

关键词：

Urease activity; Urea; Surface-enhanced Raman scattering; Healthcare; RAPID DETECTION; SERS ASSAY; DISEASE; SPECTROSCOPY;

D O I：

10.1016/j.bios.2018.12.059

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The detection of urease activity in the oral cavity is considered to be an efficient way to prevent dental caries and also to screen for helicobacter pylori infection. Herein, a rapid surface enhanced Raman scattering (SERS) method is proposed to determine the activity of urease by using inositol hexaphosphate (IP6) stabilized silver nanoparticles (AgNPs@IP6) as an efficient SERS-active substrate. The determination was achieved by monitoring the SERS peak intensity of urea at 1003 cm(-1). With urease increase, the response of urea at 1003 cm-1 decreases gradually, indicating the two has good correlation. A linear relationship between the absolute value of signal drop and urease concentration is observed in a range from 2.35 to 37.5 mu g/mL. In addition, the rapid SERS method was used to evaluate the activity of urease in real sample of saliva without any pretreatment, exhibiting a promising potential for biomedical application.

引用

页码：24 / 28

页数：5

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