Verification of the Role of MlrC in Microcystin Biodegradation by Studies Using a Heterologously Expressed Enzyme

被引：30
作者：
Dziga, Dariusz [2 ]
Wasylewski, Marcin [1 ]
Szetela, Adam [2 ]
Bochenska, Oliwia [3 ]
Wladyka, Benedykt [3 ]
机构：
[1] Jagiellonian Univ, Fac Biochem Biophys & Biotechnol, Dept Cell Biol, PL-30387 Krakow, Poland
[2] Jagiellonian Univ, Fac Biochem Biophys & Biotechnol, Dept Plant Physiol & Dev, PL-30387 Krakow, Poland
[3] Jagiellonian Univ, Fac Biochem Biophys & Biotechnol, Dept Analyt Biochem, PL-30387 Krakow, Poland
来源：
CHEMICAL RESEARCH IN TOXICOLOGY | 2012年 / 25卷 / 06期
关键词：
BACTERIAL-DEGRADATION; MICROBIAL-DEGRADATION; GENE-CLUSTER; LR;
D O I：
10.1021/tx300174e
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
The MlrC protein from Sphingomonas ACM-3962 strain was heterologously expressed in Escherichia coli strain BL21(DE3) and purified to investigate participation of this enzyme in the biodegradation of two microcystin variants. In contrast with previous reports, our results indicated that MlrC cleaves linear microcystins, thus shedding new light on the role of MlrC enzyme in microcystin biodegradation.
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页码：1192 / 1194
页数：3
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