A new disruption vector (pDHO) to obtain heterothallic strains from both Saccharomyces cerevisiae and Saccharomyces pastorianus

被引：2

作者：

Blasco, Lucia ^{[1
]}

Veiga-Crespo, Patricia ^{[3
]}

Vinas, Miguel ^{[4
]}

Villa, Tomas G. ^{[1
,2
]}

机构：

[1] Univ Santiago de Compostela, Fac Pharm, Dept Microbiol, Santiago De Compostela 15782, Spain

[2] Univ Santiago de Compostela, Fac Pharm, Sch Biotechnol, Santiago De Compostela 15782, Spain

[3] Univ Basque Country, Fac Med, Dept Cellular Biol & Histol, Biscay, Spain

[4] Univ Barcelona, IDIBELL, Sch Med, Dept Pathol & Expt Therapeut, Barcelona, Spain

来源：

INTERNATIONAL MICROBIOLOGY | 2011年 / 14卷 / 04期

关键词：

Saccharomyces cerevisiae; Saccharomyces pastorianus; homothallic; heterothallic; gene deletion; GENE DISRUPTION; YEAST; GENOME; WINE; WINEMAKING; EVOLUTION; HYBRID;

D O I：

10.2436/20.1501.01.149

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Yeasts are responsible for several traits in fermented beverages, including wine and beer, and their genetic manipulation is often necessary to improve the quality of the fermentation product. Improvement of wild-type strains of Saccharomyces cerevisiae and Saccharomyces pastorianus is difficult due to their homothallic character and variable ploidy level. Homothallism is determined by the HO gene in S. cerevisiae and the Sc-HO gene in S. pastorianus. In this work, we describe the construction of an HO disruption vector (pDHO) containing an HO disruption cassette and discuss its use in generating heterothallic yeast strains from homothallic Saccharomyces species. [Int Microbiol 2011; 14(4):201-206]

引用

页码：201 / 206

页数：6

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