Complexation-induced resolution enhancement of 3D-printed hydrogel constructs

被引：189

作者：

Gong, Jiaxing ^{[1
,2
,3
]}

Schuurmans, Carl C. L. ^{[1
,4
,5
]}

van Genderen, Anne Metje ^{[1
,5
]}

Cao, Xia ^{[1
]}

Li, Wanlu ^{[1
]}

Cheng, Feng ^{[1
]}

He, Jacqueline Jialu ^{[1
]}

Lopez, Arturo ^{[1
]}

Huerta, Valentin ^{[1
]}

Manriquez, Jennifer ^{[1
]}

Li, Ruiquan ^{[6
]}

Li, Hongbin ^{[1
]}

Delavaux, Clement ^{[1
]}

Sebastian, Shikha ^{[1
]}

Capendale, Pamela E. ^{[1
,4
,5
]}

Wang, Huiming ^{[2
,3
]}

Xie, Jingwei ^{[6
]}

Yu, Mengfei ^{[2
,3
]}

Masereeuw, Rosalinde ^{[5
]}

Vermonden, Tina ^{[4
]}

Zhang, Yu Shrike ^{[1
]}

机构：

[1] Harvard Med Sch, Brigham & Womens Hosp, Dept Med, Div Engn Med, 65 Landsdowne St, Cambridge, MA 02139 USA

[2] Zhejiang Univ, Sch Med, Affiliated Stomatol Hosp, Hangzhou 310003, Zhejiang, Peoples R China

[3] Key Lab Oral Biomed Res Zhejiang Prov, Hangzhou 310003, Zhejiang, Peoples R China

[4] Univ Utrecht, Utrecht Inst Pharmaceut Sci UIPS, Dept Pharmaceut, Sci Life, Univ Weg 99, NL-3508 TB Utrecht, Netherlands

[5] Univ Utrecht, Utrecht Inst Pharmaceut Sci UIPS, Dept Pharmacol, Univ Weg 99, NL-3508 TB Utrecht, Netherlands

[6] Univ Nebraska Med Ctr, Dept Surg, Transplant & Holland Regenerat Med Program, 985965 Nebraska Med Ctr, Omaha, NE 68198 USA

来源：

NATURE COMMUNICATIONS | 2020年 / 11卷 / 01期

基金：

美国国家卫生研究院;

关键词：

GELATIN; COACERVATION; CHITOSAN; TISSUES;

D O I：

10.1038/s41467-020-14997-4

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Three-dimensional (3D) hydrogel printing enables production of volumetric architectures containing desired structures using programmed automation processes. Our study reports a unique method of resolution enhancement purely relying on post-printing treatment of hydrogel constructs. By immersing a 3D-printed patterned hydrogel consisting of a hydrophilic polyionic polymer network in a solution of polyions of the opposite net charge, shrinking can rapidly occur resulting in various degrees of reduced dimensions comparing to the original pattern. This phenomenon, caused by complex coacervation and water expulsion, enables us to reduce linear dimensions of printed constructs while maintaining cytocompatible conditions in a cell type-dependent manner. We anticipate our shrinking printing technology to find widespread applications in promoting the current 3D printing capacities for generating higher-resolution hydrogel-based structures without necessarily having to involve complex hardware upgrades or other printing parameter alterations.

引用

页数：14

共 38 条

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