Confocal laser endomicroscopy in gastrointestinal diseases

被引:48
作者
Goetz, Martin [2 ]
Watson, Alastair [1 ]
Kiesslich, Ralf [2 ]
机构
[1] Univ E Anglia, Fac Med & Hlth Sci, Norwich Med Sch, Norwich NR4 7TJ, Norfolk, England
[2] Univ Med Mainz, Med Klin & Poliklin, Mainz, Germany
关键词
endomicroscopy; molecular imaging; Barrett's esophagus; squamous cell cancer; gastric cancer; celiac disease; ulcerative colitis; colorectal cancer; epidermal growth factor receptor; Vascular Endothelial Growth Factor; IN-VIVO DIAGNOSIS; INTRAEPITHELIAL NEOPLASIA; FLUORESCENCE MICROSCOPY; COLLAGENOUS COLITIS; HISTOLOGY; GAPS; IDENTIFICATION; DENSITY;
D O I
10.1002/jbio.201100022
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Confocal laser endomicroscopy (CLE) is a novel endoscopic technique permitting in vivo microscopy (optical biopsies) of the gastrointestinal mucosa. CLE has been studied in a multitude of diseases of the upper and lower gastrointestinal tract, including Barrett's esophagus, gastric inflammation and cancer, celiac disease, colorectal adenoma and carcinoma, and inflammatory bowel diseases. CLE has recently evolved and been studied for bile duct and liver imaging. CLE has shown overall high accuracy and enabled smart, targeted biopsies rather than untargeted sampling. Furthermore, the availability of real time microscopic information during endoscopy has immediate impact on therapeutic decisions and guides endoscopic interventions. CLE is also a unique tool for observation of (patho-)physiologic events in their natural environment (functional imaging) and has been linked to molecular imaging of gastrointestinal neoplasia in vivo, thereby broadening our understanding of mucosal pathology in clinical and basic science. Two types of CLE systems for confocal endomicroscopy: endomicroscope (upper panel) with laser scanner integrated into the distal part of an endoscope and probe-based CLE (lower panel).
引用
收藏
页码:498 / 508
页数:11
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