Enhancement of bone formation on LBL-coated Mg alloy depending on the different concentration of BMP-2

被引:22
作者
Kim, Seo-Young [1 ,2 ,3 ]
Kim, Yu-Kyoung [1 ,2 ,3 ]
Kim, Kyung-Seon [4 ]
Lee, Kwang-Bok [5 ]
Lee, Min-Ho [1 ,2 ,3 ]
机构
[1] Chonbuk Natl Univ, Dept Dent Biomat, Sch Dent, 567 Baekje Daero, Jeonju Si 54896, Jeollabuk Do, South Korea
[2] Chonbuk Natl Univ, Inst Biodegradable Mat, Sch Dent, Inst Oral Biosci, 567 Baekje Daero, Jeonju Si 54896, Jeollabuk Do, South Korea
[3] Chonbuk Natl Univ, BK21 Plus Project, Sch Dent, 567 Baekje Daero, Jeonju Si 54896, Jeollabuk Do, South Korea
[4] Jeonju Kijeon Coll, Dept Dent Hyg, 267 Jeonjucheonseo Ro, Jeonju Si 54989, Jeollabuk Do, South Korea
[5] Chonbuk Natl Univ, Med Sch, Dept Orthoped Surg, Res Inst Clin Med,Biomed Res Inst,Chonbuk Natl Un, Gungiro 20, Jeonju Si 561180, Jeollabuk Do, South Korea
基金
新加坡国家研究基金会;
关键词
MAO coating; Hydrothermal treatment; Bone morphogenic protein-2; Corrosion test; Cytotoxicity test; In-vivo test; MORPHOGENETIC PROTEIN-2; IN-VIVO; BIODEGRADABLE MAGNESIUM; TITANIUM SURFACES; POROUS TITANIUM; MC3T3-E1; CELLS; GROWTH-FACTOR; IMPLANTS; IMMOBILIZATION; EXPRESSION;
D O I
10.1016/j.colsurfb.2018.09.061
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
To control the bio-absorption rate of Mg alloy and improve its biocompatibility, a BMP-2 of various concentrations (20, 50, and 100 ng/mL) was immobilized in the carrier layer formed by micro-arc oxidation + hydrothermal treatment on the surface of Mg alloy. Their morphological and chemical properties were evaluated by FE-SEM with EDS and XRD. The electrochemical corrosion behavior was examined, and the cytotoxicity was assessed by WST and ALP assay. After in vivo test during 2 and 4 weeks in the rat tibia, histological observation was performed. In the carrier layer, calcium phosphate ceramic and oxide layers (MgO and Mg(OH)(2)) were formed. The layers enhanced the corrosion resistance. It was maintained even after loading the BMP-2. In the BMP-2-loaded group, the proliferation and differentiation of the osteoblast cells were promoted by the continuous release of the BMP-2. After implantation for 2 weeks, all BMP-2-loaded groups showed better biodegradation rate and osseointegration than the control group. After 4 weeks, the group with 50 ng/mL of BMP-2 exhibited the slowest biodegradation rate of all the BMP-2-loaded groups. This effected on the enhancement of new bone formation and the stable bone growth. Therefore, for the short term of implantation, this double coating method could be applied onto biodegradable Mg metal as an orthopedics and dental implant. For the long term of implantation over 4 weeks, to treat Mg implant with 50 ng/mL of BMP-2 was determined to be optimal.
引用
收藏
页码:437 / 446
页数:10
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