A malaria gametocytocidal assay using oxidoreduction indicator, alamarBlue

被引：76

作者：

Tanaka, Takeshi Q. ^{[1
]}

Williamson, Kim C. ^{[1
,2
]}

机构：

[1] NIAID, Lab Malaria & Vector Res, NIH, Rockville, MD 20892 USA

[2] Loyola Univ, Dept Biol, Chicago, IL 60660 USA

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 2011年 / 177卷 / 02期

基金：

美国国家卫生研究院;

关键词：

AlamarBlue; Drug screening; Gametocyte; Plasmodium falciparum; PLASMODIUM-FALCIPARUM GAMETOCYTES; ARTEMISININ COMBINATION THERAPIES; FLOW-CYTOMETRY; RESISTANCE; MEFLOQUINE; AFRICA;

D O I：

10.1016/j.molbiopara.2011.02.005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Efforts to move from malaria control to eradication will require new approaches to block malaria transmission, such as the development of anti-malarial drugs with gametocytocidal activity. Here fluorescent oxidoreduction indicator alamarBlue is used to develop a screen for gametocyte viability. The fluorescent signal increases linearly with gametocyte number (R(2) = 0.99) and determination of the IC(50) of epoxomicin demonstrated the assay was reproducible and sensitive (IC(50) 2.16 +/- 0.57 nM, Z'-factor 0.81 +/- 0.01). Six anti-malarials were also tested and at 10 mu M only primaquine and dihydroartemisinin (DHA) had gametocytocidal activity. This new assay provides an important tool to efficiently screen compounds for gametocytocidal activity. Published by Elsevier B.V.

引用

页码：160 / 163

页数：4

共 20 条

[1]

BAIRD JK, 2010, TRENDS PARASITOL

[2] Trioxaquines are new antimalarial agents active on all erythrocytic forms, including gametocytes [J].

Benoit-Vical, Francoise ;

Lelievre, Joel ;

Berry, Antoine ;

Deymier, Caroline ;

Dechy-Cabaret, Odile ;

Cazelles, Jerome ;

Loup, Christophe ;

Robert, Anne ;

Magnaval, Jean-Francois ;

Meunier, Bernard .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2007, 51 (04) :1463-1472

[3] Changes in the Treatment Responses to Artesunate-Mefloquine on the Northwestern Border of Thailand during 13 Years of Continuous Deployment [J].

Carrara, Verena Ilona ;

Zwang, Julien ;

Ashley, Elizabeth A. ;

Price, Ric N. ;

Stepniewska, Kasia ;

Barends, Marion ;

Brockman, Alan ;

Anderson, Tim ;

McGready, Rose ;

Phaiphun, Lucy ;

Proux, Stephane ;

van Vugt, Michele ;

Hutagalung, Robert ;

Lwin, Khin Maung ;

Phyo, Aung Pyae ;

Preechapornkul, Piyanuch ;

Imwong, Mallika ;

Pukrittayakamee, Sasithon ;

Singhasivanon, Pratap ;

White, Nicholas J. ;

Nosten, Francois .

PLOS ONE, 2009, 4 (02)

[4]

CHEN PQ, 1994, CHINESE MED J-PEKING, V107, P709

[5] Flow cytometry for the evaluation of anti-plasmodial activity of drugs on Plasmodium falciparum gametocytes [J].

Chevalley, Severine ;

Coste, Agnes ;

Lopez, Alexandrine ;

Pipy, Bernard ;

Valentin, Alexis .

MALARIA JOURNAL, 2010, 9

[6] The Proteasome Inhibitor Epoxomicin Has Potent Plasmodium falciparum Gametocytocidal Activity [J].

Czesny, Beata ;

Goshu, Samrawit ;

Cook, James L. ;

Williamson, Kim C. .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2009, 53 (10) :4080-4085

[7] Thousands of chemical starting points for antimalarial lead identification [J].

Gamo, Francisco-Javier ;

Sanz, Laura M. ;

Vidal, Jaume ;

de Cozar, Cristina ;

Alvarez, Emilio ;

Lavandera, Jose-Luis ;

Vanderwall, Dana E. ;

Green, Darren V. S. ;

Kumar, Vinod ;

Hasan, Samiul ;

Brown, James R. ;

Peishoff, Catherine E. ;

Cardon, Lon R. ;

Garcia-Bustos, Jose F. .

NATURE, 2010, 465 (7296) :305-U56

[8] Chemical genetics of Plasmodium falciparum [J].

Guiguemde, W. Armand ;

Shelat, Anang A. ;

Bouck, David ;

Duffy, Sandra ;

Crowther, Gregory J. ;

Davis, Paul H. ;

Smithson, David C. ;

Connelly, Michele ;

Clark, Julie ;

Zhu, Fangyi ;

Jimenez-Diaz, Maria B. ;

Martinez, Maria S. ;

Wilson, Emily B. ;

Tripathi, Abhai K. ;

Gut, Jiri ;

Sharlow, Elizabeth R. ;

Bathurst, Ian ;

El Mazouni, Farah ;

Fowble, Joseph W. ;

Forquer, Isaac ;

McGinley, Paula L. ;

Castro, Steve ;

Angulo-Barturen, Inigo ;

Ferrer, Santiago ;

Rosenthal, Philip J. ;

DeRisi, Joseph L. ;

Sullivan, David J., Jr. ;

Lazo, John S. ;

Roos, David S. ;

Riscoe, Michael K. ;

Phillips, Margaret A. ;

Rathod, Pradipsinh K. ;

Van Voorhis, Wesley C. ;

Avery, Vicky M. ;

Guy, R. Kiplin .

NATURE, 2010, 465 (7296) :311-315

[9] Complete in vitro maturation of Plasmodium falciparum gametocytes [J].

Ifediba, T. ;

Vanderberg, J. P. .

NATURE, 1981, 294 (5839) :364-366

[10] A comparative study of a flow-cytometry-based assessment of in vitro Plasmodium falciparum drug sensitivity [J].

Karl, Stephan ;

Wong, Rina P. M. ;

St Pierre, Tim G. ;

Davis, Timothy M. E. .

MALARIA JOURNAL, 2009, 8

← 1 2 →