A cytological indicator allows rapid assessment of microspore maturity, leading to improved in vitro anther response in Indica rice (Oryza sativa L.)

被引:10
作者
Mayakaduwa, D. M. R. G. [1 ]
Silva, T. D. [1 ]
机构
[1] Univ Colombo, Dept Plant Sci, Colombo 03, Sri Lanka
基金
美国国家科学基金会;
关键词
Anther culture; Callus induction; Nuclear division; Starch deposition; Microspore maturity; CALLUS INDUCTION; PLANT-REGENERATION; CULTURE; HAPLOIDS; CULTIVARS; HYBRIDS;
D O I
10.1007/s11627-017-9855-0
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The inadequate response of Indica rice (Oryza sativa L.) to anther culture hinders the utility of this technique for rice crop improvement. This study focused on intensifying the in vitro anther response of selected varieties from Sri Lankan germplasm by precisely targeting the most effective maturity stage of microspores for culture. Analysis of five varieties responsive to anther culture ("At 303", "Bw 272-6B", "Dahanala", "Dikwee", and "Herath Banda"), revealed that the microspore development stage at the time of culture was crucial for fine-tuning the callus induction response. The external guide used to determine microspore maturity which was the internode distance between the last two leaves of the panicle, could not be applied equally for all rice varieties. Anther response could be improved by, at least, four- to sixfold by precisely targeting anthers with late uninucleate microspores for culture. Close correlation was demonstrated between the stage of nuclear division in the microspores and the degree of starch accumulation. Therefore, the degree of starch deposition in the microspores can be used as an effective cytological marker, which can be applied more rapidly and reliably to assess microspore maturity, in place of the internode distance.
引用
收藏
页码:591 / 597
页数:7
相关论文
共 18 条
  • [1] Effect of spikelet position on rice anther culture efficiency
    Afza, R
    Shen, M
    Zapata-Arias, FJ
    Xie, JH
    Fundi, HK
    Lee, KS
    Bobadilla-Mucino, E
    Kodym, A
    [J]. PLANT SCIENCE, 2000, 153 (02) : 155 - 159
  • [2] Bagheri Nadali, 2009, Biharean Biologist, V3, P119
  • [3] Anther culture of recalcitrant indica x Basmati rice hybrids - Anther culture of indica rice hybrids
    Bishnoi, U
    Jain, RK
    Rohilla, JS
    Chowdhury, VK
    Gupta, KR
    Chowdhury, JB
    [J]. EUPHYTICA, 2000, 114 (02) : 93 - 101
  • [4] An efficient procedure for embryogenic callus induction and double haploid plant regeneration through anther culture of Thai aromatic rice (Oryza sativa L. subsp indica)
    Cha-um, Suriyan
    Srianan, Bootsaya
    Pichakum, Aussanee
    Kirdmanee, Chalermpol
    [J]. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT, 2009, 45 (02) : 171 - 179
  • [5] Chu CC., 1978, PROC S PLANT TISSUE, P43
  • [6] Datta SK, 2005, CURR SCI INDIA, V89, P1870
  • [7] Haploids in flowering plants: origins and exploitation
    Dunwell, Jim M.
    [J]. PLANT BIOTECHNOLOGY JOURNAL, 2010, 8 (04) : 377 - 424
  • [8] Anther culture for haploid and doubled haploid production
    Germana, Maria Antonietta
    [J]. PLANT CELL TISSUE AND ORGAN CULTURE, 2011, 104 (03) : 283 - 300
  • [9] IMPROVED RATE OF CALLUS INDUCTION FROM RICE ANTHER CULTURE FOLLOWING MICROSCOPIC STAGING OF MICROSPORES IN IRON ALUM-HEMATOXYLIN
    GUPTA, HS
    BORTHAKUR, DN
    [J]. THEORETICAL AND APPLIED GENETICS, 1987, 74 (01) : 95 - 99
  • [10] Selection of interspecific hybrids for anther culture of indica rice
    He, T.
    Yang, Y.
    Tu, S. B.
    Yu, M. Q.
    Li, X. F.
    [J]. PLANT CELL TISSUE AND ORGAN CULTURE, 2006, 86 (02) : 271 - 277