Full characterization and cytotoxic activity of new silver(I) and copper(I) helicates with quaterpyridine

被引：20

作者：

Adamski, Ariel ^{[1
]}

Fik, Marta A. ^{[1
]}

Kubicki, Maciej ^{[1
]}

Hnatejko, Zbigniew ^{[1
]}

Gurda, Dorota ^{[2
]}

Fedoruk-Wyszomirska, Agnieszka ^{[2
]}

Wyszko, Eliza ^{[2
]}

Kruszka, Dariusz ^{[3
]}

Dutkiewicz, Zbigniew ^{[3
]}

Patroniak, Violetta ^{[1
]}

机构：

[1] Adam Mickiewicz Univ, Fac Chem, Umultowska 89B, PL-61614 Poznan, Poland

[2] Polish Acad Sci, Inst Bioorgan Chem, Noskowskiego 12-14, PL-61704 Poznan, Poland

[3] Poznan Univ Med Sci, Dept Chem Technol Drugs, Grunwaldzka 6, PL-60780 Poznan, Poland

来源：

NEW JOURNAL OF CHEMISTRY | 2016年 / 40卷 / 09期

关键词：

CRYSTAL-STRUCTURES; DNA-BINDING; SUPRAMOLECULAR COMPLEXES; SCHIFF-BASE; LIGANDS; DINUCLEAR; CHEMISTRY; LUMINESCENCE; MONONUCLEAR; CHIRALITY;

D O I：

10.1039/c5nj03601a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The self-assembly of 6,6 ''''-dimethyl-2,2';6',2 '';6 '',2'''-quaterpyridine L with tetrahedral ions of silver(I) and copper(I) leads to the formation of dinuclear double stranded helicates 1-4. H-1 NMR studies confirm the stability of each helicate both in 2% aqueous DMSO solution, which plays the role of a medium in biological studies, and in the cell extract solution. The cytotoxic properties of complexes were examined on two cancerous cell lines: HeLa and T-47D as well as on the non-neoplastic fibroblasts HaCaT by using the MTT assay method. The IC50 values of cisplatin towards cancerous cell lines are rather comparable. However, the trend of cancerous/healthy cell selectivity is not maintained. One may assume a different mechanism of action. Moreover, it is possible to distinguish the cell death pathway triggered by Ag(I) and Cu(I) helicates presented in this study. Flow cytometry, EtBr displacement titrations, CD titrations, DNA melting experiments and DFT calculations were used to characterize the type of interaction and the mechanism of cytotoxic action.

引用

页码：7943 / 7957

页数：15

共 63 条

[1] Absorption spectra, luminescence properties and electrochemical behavior of Mn(II), Fe(III) and Pt(II) complexes with quaterpyridine ligand [J].