Centrifugation-Controlled Thermal Convection and Its Application to Rapid Microfluidic Polymerase Chain Reaction Devices

被引:12
作者
Saito, Masato [1 ]
Takahashi, Kazuya [1 ]
Kiriyama, Yuichiro [1 ]
Espulgar, Wilfred Villariza [1 ]
Aso, Hiroshi [2 ]
Sekiya, Tadanobu [2 ]
Tanaka, Yoshikazu [2 ]
Sawazumi, Tsuneo [2 ]
Furui, Satoshi [3 ]
Tamiya, Eiichi [1 ]
机构
[1] Osaka Univ, Grad Sch Engn, Dept Appl Phys, 2-1 Yamadaoka, Suita, Osaka 5650871, Japan
[2] Konica Minolta Inc, Chiyoda Ku, JP Tower,2-7-2 Marunouchi, Tokyo 1007015, Japan
[3] NARO, Food Res Inst, Food Entomol Unit, 2-1-12 Kannondai, Tsukuba, Ibaraki 3058642, Japan
关键词
ENZYMATIC AMPLIFICATION; ANALYSIS SYSTEMS; DNA; PCR; CHIP; FLOW;
D O I
10.1021/acs.analchem.7b03107
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Here, we report the developed cydo olefin polymer (COP) microfluidic chip on a fabricated rotating heater stage that utilizes centrifugation-assisted thermal cycle in a ring-structured microchannel for polymerase chain reaction (PCR). The PCR solution could be driven by thermal convection and continuously exchanged high/low temperatures: in a ring structured microchannel without the use of typical syringe pump. More importantly, the flow, rate was controlled by the relative gravitational acceleration only. The platform enables amplification within 10 min at 5G and has a detection limit of 70.5 pg/channel DNA concentration (beta-actin, 295 bp). The current rotating system is capable of testing four different samples in parallel: The microfluidic chip can be preloaded with the PCR premix solution for on-site utility, and, with all of the features integrated to the system, the test can be conducted without the need for specialized laboratory and trained laboratory staff. In addition, this innovative chemical reaction technique has the potential to be utilized in other micromixing applications.
引用
收藏
页码:12797 / 12804
页数:8
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