Archaeal Poly (3-hydroxybutyrate) Polymer Production from Glycerol: Optimization by Taguchi Methodology

被引:6
作者
Taran, Mojtaba [1 ]
Azizi, Elham [1 ]
Taran, Shahein [2 ]
Asadi, Nadia [3 ]
机构
[1] Razi Univ, Dept Biol, Fac Sci, Kermanshah, Iran
[2] Univ Tehran, Fac Phys Educ & Sport Sci, Tehran, Iran
[3] Kermanshah Univ Med Sci, Fac Med, Kermanshah, Iran
关键词
Archaea; Poly (3-hydroxybutyrate); Glycerol; Taguchi methodology; PROPIONIC-ACID; CARBON SOURCE; FERMENTATION; BATCH; POLYHYDROXYALKANOATES; WASTES; COLI; PHB;
D O I
10.1007/s10924-011-0327-z
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
In this study the possibility of poly (3-hydroxybutyrate) production from glycerol was investigated and optimized by Halorcula sp. IRU1, a novel archaea isolated from Urmia lake, Iran in batch experiments. Using Taguchi methodology, three important independent parameters (glycerol, yeast extract and KH2PO4) were evaluated for their individual and interactive effects on poly (3-hydroxybutyrate) production. It was shown that the glycerol concentration was the most significant factor affecting the yield of poly (3-hydroxybutyrate). The optimum factor levels were a glycerol concentration of 8% (v/v), yeast extract 0.8% (w/v) and KH2PO4 0.002% (w/v). The predicted value obtained for poly (3-hydroxybutyrate) production under these conditions was about 81.87%. We can conclude that Haloarcula sp. IRU1 has a high potential for synthesis of poly (3-hydroxybutyrate) from glycerol.
引用
收藏
页码:750 / 754
页数:5
相关论文
共 29 条
[1]  
Arun A, 2006, AFR J BIOTECHNOL, V5, P1524
[2]  
AZEGAR AL, 2003, ANN MICROSC, V3, P221
[3]   Propionic acid fermentation from glycerol: Comparison with conventional substrates [J].
Barbirato, F ;
Chedaille, D ;
Bories, A .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1997, 47 (04) :441-446
[4]   Fermentation of glycerol by Clostridium pasteurianum -: batch and continuous culture studies [J].
Biebl, H .
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 2001, 27 (01) :18-26
[5]   Glycerol fermentation with Propionibacteria and optimisation of the production of propionic acid [J].
Bories, A ;
Himmi, E ;
Jauregui, JJA ;
Pelayo-Ortiz, C ;
Gonzales, VA .
SCIENCES DES ALIMENTS, 2004, 24 (02) :121-135
[6]   The production of polyhydroxybutyrate by Methylobacterium rhodesianum and Ralstonia eutropha in media containing glycerol and casein hydrolysates [J].
Bormann, EJ ;
Roth, M .
BIOTECHNOLOGY LETTERS, 1999, 21 (12) :1059-1063
[7]   Polyhydroxyalkanoates, biopolyesters from renewable resources: Physiological and engineering aspects [J].
Braunegg, G ;
Lefebvre, G ;
Genser, KF .
JOURNAL OF BIOTECHNOLOGY, 1998, 65 (2-3) :127-161
[8]   Glycerol: A promising and abundant carbon source for industrial microbiology [J].
da Silva, Gervasio Paulo ;
Mack, Matthias ;
Contiero, Jonas .
BIOTECHNOLOGY ADVANCES, 2009, 27 (01) :30-39
[9]   Effects of granule-associated protein PhaP on glycerol-dependent growth and polymer production in poly(3-hydroxybutyrate)-producing Eschetichia coli [J].
de Almeida, Alejandra ;
Nikel, Pablo I. ;
Giordano, Andrea M. ;
Pettinari, M. Julia .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2007, 73 (24) :7912-7916
[10]  
Fusun T., 2000, TURK J MED SCI, V30, P535