Biosynthesis of Oleyl Oleate Wax Ester by Non-commercial Lipase

被引：11

作者：

Lopes, Danielle Branta ^{[1
]}

Teixeira Duarte, Marta Cristina ^{[2
]}

Macedo, Gabriela Alves ^{[1
]}

机构：

[1] Univ Estadual Campinas, Dept Food Sci, BR-13083862 Campinas, SP, Brazil

[2] Univ Estadual Campinas, Multidisciplinary Ctr Biol & Agr Chem, BR-13140000 Paulinia, SP, Brazil

来源：

FOOD SCIENCE AND BIOTECHNOLOGY | 2011年 / 20卷 / 05期

关键词：

lipase; Rhizopus spp; wax ester; lipase-catalyzed esterification; oleyl oleate; ENZYMATIC-SYNTHESIS; IMMOBILIZED LIPASE; OPTIMIZATION; OIL;

D O I：

10.1007/s10068-011-0166-7

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Oleyl oleate can be considered a synthetic analogue of jojoba oil. This one has been the main natural source of wax esters for commercial applications since the global ban on whale hunting. Lipase catalyzed production of this ester was carried out using oleic acid and oleyl alcohol in a solvent-free system. Lipase from Rhizopus sp. CBMAI 1127 was used as the biocatalyst and commercial enzyme Lipozyme TL IM (R) was used to compare results. The acid/alcohol molar ratio showed significant effects for both lipases and the amount of enzyme had a significant effect just for Lipozyme TL IM (R). The rate of the esterification reaction using lipase from Rhizopus sp. CBMAI 1127 was very similar to that obtained with commercial lipase. The present study also evaluated antimicrobial and emulsifying properties of this ester. Emulsifying capacity of the synthesized oleyl oleate was lower than Tween 80 and sodium dodecyl sulfate, and no antimicrobial activity was observed.

引用

页码：1203 / 1209

页数：7

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