Nicotine induces neutrophil extracellular traps

被引：122

作者：

Hosseinzadeh, Ava ^{[1
,2
,3
]}

Thompson, Paul R. ^{[4
]}

Segal, Brahm H. ^{[5
,6
,7
]}

Urban, Constantin F. ^{[1
,2
,3
]}

机构：

[1] Umea Univ, Dept Clin Microbiol, Umea, Sweden

[2] UCMR, Umea, Sweden

[3] Lab Mol Infect Med, Umea, Sweden

[4] Univ Massachusetts, Sch Med, Dept Biochem & Mol Pharmacol, Worcester, MA USA

[5] Roswell Pk Canc Inst, Dept Med, Buffalo, NY 14263 USA

[6] Roswell Pk Canc Inst, Dept Immunol, Buffalo, NY 14263 USA

[7] Univ Buffalo, Dept Med, Jacobs Sch Med & Biomed Sci, Buffalo, NY USA

来源：

JOURNAL OF LEUKOCYTE BIOLOGY | 2016年 / 100卷 / 05期

基金：

瑞典研究理事会; 美国国家卫生研究院;

关键词：

tobacco; protein kinase B; ROS; nicotinic acetylcholine receptor; CIGARETTE-SMOKING; MITOCHONDRIAL-DNA; SPONTANEOUS DEATH; TOBACCO SMOKING; NET FORMATION; IN-VIVO; RELEASE; DISEASE; NETOSIS; MECHANISMS;

D O I：

10.1189/jlb.3AB0815-379RR

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

NETs serve to ensnare and kill microbial pathogens. However, NETs can at the same time contribute to tissue damage and excessive inflammation. Nicotine is a major toxic agent and has been associated with exacerbated inflammatory diseases. The current study aimed at investigating the role of nicotine, the addictive component of tobacco and electronic cigarettes, on triggering NET formation. We report that nicotine induces neutrophils to release NETs in a dose-dependent manner. Nicotine-induced NET formation is mediated via nicotine acetylcholine receptors, depends on Akt and PAD4 activation, but is Nox2-independent, as demonstrated by pharmacological inhibition of Nox2 and by use of Nox2-deficient mouse neutrophils. These findings demonstrate that nicotine induces NETs, which may in turn contribute to smoking-related diseases.

引用

页码：1105 / 1112

页数：8

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