Biolistic infection of cassava using cloned components of Indian cassava mosaic virus

被引：15
|
作者：
Rothenstein, D
Briddon, RW
Haible, D
Stanley, J
Frischmuth, T
Jeske, H
机构：
[1] Univ Stuttgart, Dept Mol Biol & Plant Virol, D-70550 Stuttgart, Germany
[2] John Innes Ctr Plant Sci Res, Dept Dis & Stress Biol, Norwich NR4 7UH, Norfolk, England
来源：
ARCHIVES OF VIROLOGY | 2005年 / 150卷 / 08期
关键词：
D O I：
10.1007/s00705-005-0520-2
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Cassava mosaic disease (CMD) is a major constraint to cassava production in Africa and Asia. Of the two begomoviruses associated with CMD on the Indian subcontinent, Indian cassava mosaic virus (ICMV) and Sri Lankan cassava mosaic virus, only the latter has been successfully reintroduced into cassava to resolve the aetiology of the disease. Here, we report the complete nucleotide sequence of an ICMV isolate from Maharashtra (ICMV-[Mah2]), central India. Biolistic inoculation of the cloned components produced a systemic infection and typical mosaic symptoms in cassava, thereby fulfilling Koch's postulates. The availability of infectious clones will provide a valuable tool to screen new cassava cultivars for disease resistance under defined conditions.
引用
收藏
页码：1669 / 1675
页数：7
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