Novel acetylated α-cyclosophorotridecaose produced by Ralstonia solanacearum

被引:6
作者
Cho, Eunae [1 ]
Lee, Sanghoo [1 ]
Jung, Seunho [1 ]
机构
[1] Konkuk Univ, Dept Biosci & Biotechnol, Bio Mol Informat Ctr, Seoul 143701, South Korea
关键词
acetylated alpha-cyclosophorotridecaose; periplasmic cyclic glucan; Ralstonia solanacearum; structural analysis;
D O I
10.1016/j.carres.2008.01.023
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
alpha-Cyclosophorotridecaose (alpha-C13) produced by Ralstonia solanacearum is isolated by trichloroacetic acid treatment and subjected to various chromatographic techniques. Here, we report for the first time that R. solanacearum produces acetylated alpha-C13. Structural analyses of the acetylated alpha-C13 were performed with 1D or 2D NMR spectroscopy, MALDI-TOF MS and HPLC. The results show that the alpha-C13 is substituted by mainly one acetyl residue at the C-6 position of the glucose unit. (C) 2008 Elsevier Ltd. All rights reserved.
引用
收藏
页码:912 / 918
页数:7
相关论文
共 21 条
[1]   Detection and visualization of the major acidic exopolysaccharide of Ralstonia solancearum and its role in tomato root infection and vascular colonization [J].
Araud-Razou, I ;
Vasse, J ;
Montrozier, H ;
Etchebar, C ;
Trigalet, A .
EUROPEAN JOURNAL OF PLANT PATHOLOGY, 1998, 104 (08) :795-809
[2]   The opgGIH and opgC genes of Rhodobacter sphaeroides form an operon that controls backbone synthesis and succinylation of osmoregulated periplasmic glucans [J].
Cogez, V ;
Gak, E ;
Puskas, A ;
Kaplan, S ;
Bohin, JP .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 2002, 269 (10) :2473-2484
[3]   Osmoregulated periplasmic glucans of Erwinia chrysanthemi [J].
Cogez, V ;
Talaga, P ;
Lemoine, J ;
Bohin, JP .
JOURNAL OF BACTERIOLOGY, 2001, 183 (10) :3127-3133
[4]   GENETIC-EVIDENCE THAT EXTRACELLULAR POLYSACCHARIDE IS A VIRULENCE FACTOR OF PSEUDOMONAS-SOLANACEARUM [J].
DENNY, TP ;
BAEK, SR .
MOLECULAR PLANT-MICROBE INTERACTIONS, 1991, 4 (02) :198-206
[5]  
DEORGAMBIDE G, 1991, BIOCHEM, V266, P8312
[6]   INTERACTION OF PSEUDOMONAS-SOLANACEARUM LIPOPOLYSACCHARIDE AND EXTRACELLULAR POLYSACCHARIDE WITH AGGLUTININ FROM POTATO-TUBERS [J].
DUVICK, JP ;
SEQUEIRA, L .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1984, 48 (01) :192-198
[8]  
HAYWARD AC, 1995, PATHOGENESIS HOST SP, V1, P139
[9]   THE PRODUCTION OF ACIDIC, O-ACYLATED CYCLOSOPHORANS [CYCLIC (1-]2)-BETA-D-GLUCANS] BY AGROBACTERIUM AND RHIZOBIUM SPECIES [J].
HISAMATSU, M ;
YAMADA, T ;
HIGASHIURA, T ;
IKEDA, M .
CARBOHYDRATE RESEARCH, 1987, 163 (01) :115-122
[10]   Structural analyses of novel glycerophosphorylated α-cyclosophorohexadecaoses isolated from X. campestris pv. campestris [J].
Jung, Y ;
Park, H ;
Cho, E ;
Jung, S .
CARBOHYDRATE RESEARCH, 2005, 340 (04) :673-677