Osteogenic Treatment Initiating a Tissue-Engineered Cartilage Template Hypertrophic Transition

被引:6
作者
Fu, J. Y. [1 ]
Lim, S. Y. [1 ]
He, P. F. [1 ]
Fan, C. J. [1 ]
Wang, D. A. [1 ]
机构
[1] Nanyang Technol Univ, Sch Chem & Biomed Engn, Div Bioengn, 70 Nanyang Dr,N1-3-B2-13, Singapore 637457, Singapore
关键词
Cartilage hypertrophy; Endothelial progenitor cells; Tissue engineering; Cartilage template; ENDOTHELIAL PROGENITOR CELLS; MESENCHYMAL STEM-CELLS; ENDOCHONDRAL BONE-FORMATION; IN-VITRO; CHONDROCYTE HYPERTROPHY; ARTICULAR CHONDROCYTES; HYALINE CARTILAGE; GROWTH-PLATE; ANGIOGENESIS; REGENERATION;
D O I
10.1007/s10439-016-1615-5
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Hypertrophic chondrocytes play a critical role in endochondral bone formation as well as the progress of osteoarthritis (OA). An in vitro cartilage hypertrophy model can be used as a platform to study complex molecular mechanisms involved in these processes and screen new drugs for OA. To develop an in vitro cartilage hypertrophy model, we treated a tissue-engineered cartilage template, living hyaline cartilaginous graft (LhCG), with osteogenic medium for hypertrophic induction. In addition, endothelial progenitor cells (EPCs) were seeded onto LhCG constructs to mimic vascular invasion. The results showed that osteogenic treatment significantly inhibited the synthesis of endostatin in LhCG constructs and enhanced expression of hypertrophic marker-collagen type X (Col X) and osteogenic markers, as well as calcium deposition in vitro. Upon subcutaneous implantation, osteogenic medium-treated LhCG constructs all stained positive for Col X and showed significant calcium deposition and blood vessel invasion. Col X staining and calcium deposition were most obvious in osteogenic medium-treated only group, while there was no difference between EPC-seeded and non-seeded group. These results demonstrated that osteogenic treatment was of the primary factor to induce hypertrophic transition of LhCG constructs and this model may contribute to the establishment of an in vitro cartilage hypertrophy model.
引用
收藏
页码:2957 / 2970
页数:14
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