1H NMR quantitative determination of photosynthetic pigments from green beans (Phaseolus vulgaris L.)

被引：27

作者：

Valverde, Juan ^{[1
]}

This, Herve ^{[1
]}

机构：

[1] INRA, Inst Sci Vivant & Environm, UMR 214, Equipe INRA Gastronom Mol, F-75005 Paris, France

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2008年 / 56卷 / 02期

关键词：

Phaseolus vulgaris L; crude extracts; plant pigments; H-1; NMR;

D O I：

10.1021/jf070277j

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Using H-1 nuclear magnetic resonance spectroscopy (1D and 2D), the two types of photosynthetic pigments (chlorophylls, their derivatives, and carotenoids) of "green beans" (immature pods of Phaseolus vulgaris L.) were analyzed. Compared to other analytical methods (light spectroscopy or chromatography), H-1 NMR spectroscopy is a fast analytical way that provides more information on chlorophyll derivatives (allomers and epimers) than ultraviolet-visible spectroscopy. Moreover, it gives a large amount of data without prior chromatographic separation.

引用

页码：314 / 320

页数：7

共 35 条

[1]

Belitz HD, 2004, FOOD CHEM, P1070

[2] Color improvement and metallo-chlorophyll complexes in continuous flow aseptically processed peas [J].

Canjura, FL ;

Watkins, RH ;

Schwartz, SJ .

JOURNAL OF FOOD SCIENCE, 1999, 64 (06) :987-990

[3] Sucrose, glucose, and fructose extraction in aqueous carrot root extracts prepared at different temperatures by means of direct NMR measurements [J].

Cazor, Anne ;

Deborde, Catherine ;

Moing, Annick ;

Rolin, Dominique ;

This, Herve .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2006, 54 (13) :4681-4686

[4] Anthocyanin profile of Korean cultivated kidney bean (Phaseolus vulgaris L.) [J].

Choung, MG ;

Choi, BR ;

An, YN ;

Chu, YH ;

Cho, YS .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2003, 51 (24) :7040-7043

[5] The effects of various culinary treatments on the pigment content of green beans (Phaseolus vulgaris, L.) [J].

de la Cruz-Garcia, C ;

Gonzalez-Castro, MJ ;

Oruna-Concha, MJ ;

Lopez-Hernandez, J ;

Simal-Lozano, JA ;

Simal-Gandara, J .

FOOD RESEARCH INTERNATIONAL, 1997, 30 (10) :787-791

[6] A routine method to determine the chlorophyll alpha, pheophytin alpha and beta-carotene contents of isolated Photosystem II reaction center complexes [J].

Eijckelhoff, C ;

Dekker, JP .

PHOTOSYNTHESIS RESEARCH, 1997, 52 (01) :69-73

[7] NMR OF CAROTENOIDS - NOVEL EXPERIMENTAL-TECHNIQUES [J].

ENGLERT, G .

PURE AND APPLIED CHEMISTRY, 1991, 63 (01) :59-70

[8] Metabolite profiling by one- and two-dimensional NMR analysis of complex mixtures [J].

Fan, WMT .

PROGRESS IN NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY, 1996, 28 (pt 2) :161-219

[9] Improved method to track chlorophyll degradation [J].

Gauthier-Jaques, A ;

Bortlik, K ;

Hau, J ;

Fay, LB .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2001, 49 (03) :1117-1122

[10] CAROTENOID COMPOSITION IN RAW AND COOKED SPANISH VEGETABLES [J].

GRANADO, F ;

OLMEDILLA, B ;

BLANCO, I ;

ROJASHIDALGO, E .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1992, 40 (11) :2135-2140

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