Lysobacter olei sp nov., isolated from oil-contaminated soil

Strain D-14(T), a brown-coloured, Gram-stain-negative, non-motile and rod-shaped bacterium, was isolated from oil-contaminated soil. It was able to grow at 20-40 degrees C, at pH 6.0-10.0 and at 0-1% (w/v) NaCl concentration. Based on the 16S rRNA gene sequence analysis, strain D-14(T) belonged to the genus Lysobacter and was closely related to Lysobacter caeni BUT-8(T) (99.0% sequence similarity), Lysobacter ruishenii CTN-1(T) (98.5 %), Lysobacter daejeonensis GH1-9(T) (98.2 %) and Lysobacter panacisoli CJ29(T) (97.2 %). The only respiratory quinone was ubiquinone-8. The polar lipid profile revealed the presence of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidyl-N-methylethanolamine. The predominant fatty acids of strain D-14(T) were iso-C-15 : 0, iso-C-16 : 0, summed feature 9 (iso-C-17 : 1 omega 9c and/or C-16 : 0 10-methyl), summed feature 3 (C-16 : 1 omega 7c and/or C-16 : 1 omega 6c), iso-C-14 : 0, C(11 : 0)iso 3-OH, C(15 : 1)iso F and C-16 : 0. The genomic DNA G+C content of this novel strain was 68.7 mol%. The DNA-DNA relatedness values between strain D-14(T) and L. caeni BUT-8(T), L. ruishenii CTN-1(T), L. daejeonensis GH1-9(T) and L. panacisoli CJ29(T) were 56.0, 46.3, 48.7 and 41.7 %, respectively, which fall below the threshold value of 70% for the strain to be considered as novel. The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished this strain from its closest phylogenetic neighbours. Thus, strain D-14(T) represents a novel species of the genus Lysobacter, for which the name Lysobacterolei sp. nov. is proposed. The type strain is D-14(T) (=KEMB 9005-572(T)=KACC 19173(T)=JCM 31917(T)).