A Rapid Extraction Method Combined with a Monoclonal Antibody-Based Immunoassay for the Detection of Amatoxins

被引：15

作者：

Bever, Candace S. ^{[1
]}

Hnasko, Robert M. ^{[2
]}

Cheng, Luisa W. ^{[1
]}

Stanker, Larry H. ^{[1
]}

机构：

[1] USDA ARS, Western Reg Res Ctr, Foodborne Toxin Detect & Prevent Unit, 800 Buchanan St, Albany, CA 94710 USA

[2] USDA ARS, Western Reg Res Ctr, Produce Safety & Microbiol Unit, 800 Buchanan St, Albany, CA 94710 USA

来源：

TOXINS | 2019年 / 11卷 / 12期

基金：

美国农业部;

关键词：

amatoxins; amanitins; immunoassay; monoclonal antibodies; ELISA; death cap mushrooms; AMANITA-PHALLOIDES; ALPHA-AMANITIN; BETA-AMANITIN; PHALLOTOXINS; TOXICITY; CHROMATOGRAPHY; TISSUES; TOXINS; ASSAY; FR;

D O I：

10.3390/toxins11120724

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Amatoxins (AMAs) are lethal toxins found in a variety of mushroom species. Detection methods are needed to determine the occurrence of AMAs in mushroom species suspected in mushroom poisonings. In this manuscript, we report the generation of novel monoclonal antibodies (mAbs, AMA9G3 and AMA9C12) and the development of a competitive, enzyme-linked immunosorbent assay (cELISA) that is sensitive at 1 ng mL(-1) and shows selectivity for alpha-amanitin (alpha-AMA) and gamma-amanitin (gamma-AMA), and less for beta-amanitin (beta-AMA). In order to decrease the overall time needed for analysis, the extraction procedure for mushrooms was also simplified. A rapid (1 min) extraction procedure of AMAs using solvents as simple as water alone was successfully demonstrated using Amanita mushrooms. Together, the extraction method and the mAb-based ELISA represent a simple and rapid method that readily detects AMAs extracted from mushroom samples.

引用

页数：11

共 27 条

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