Whole exome capture in solution with 3 Gbp of data

被引：120

作者：

Bainbridge, Matthew N. ^{[1
,2
]}

Wang, Min ^{[1
]}

Burgess, Daniel L. ^{[3
]}

Kovar, Christie ^{[1
]}

Rodesch, Matthew J. ^{[3
]}

D'Ascenzo, Mark ^{[3
]}

Kitzman, Jacob ^{[3
]}

Wu, Yuan-Qing ^{[1
]}

Newsham, Irene ^{[1
]}

Richmond, Todd A. ^{[3
]}

Jeddeloh, Jeffrey A. ^{[3
]}

Muzny, Donna ^{[1
]}

Albert, Thomas J. ^{[3
]}

Gibbs, Richard A. ^{[1
]}

机构：

[1] Baylor Coll Med, Human Genome Sequencing Ctr, Houston, TX 77030 USA

[2] Baylor Coll Med, Dept Struct & Computat Biol & Mol Biophys, Houston, TX 77030 USA

[3] Roche NimbleGen Inc, Madison, WI 53719 USA

来源：

GENOME BIOLOGY | 2010年 / 11卷 / 06期

关键词：

SELECTION; DISEASES; LOCI;

D O I：

10.1186/gb-2010-11-6-r62

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have developed a solution-based method for targeted DNA capture-sequencing that is directed to the complete human exome. Using this approach allows the discovery of greater than 95% of all expected heterozygous singe base variants, requires as little as 3 Gbp of raw sequence data and constitutes an effective tool for identifying rare coding alleles in large scale genomic studies.

引用

页数：8

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