Anti-proliferative effect of resveratrol, a natural component of grapes and wine, on human colonic cancer cells

被引：287
作者：
Schneider, Y
Vincent, F
Duranton, B
Badolo, L
Gossé, F
Bergmann, C
Seiler, N
Raul, F
机构：
[1] IRCAD, Lab Metab & Nutr Control Digest Oncol, ULP CJF INSERM 95 09, F-67091 Strasbourg, France
[2] IRCAD, Mol Oncol Lab, F-67091 Strasbourg, France
来源：
CANCER LETTERS | 2000年 / 158卷 / 01期
关键词：
CaCo-2; cell; polyphenol; cell cycle; ornithine decarboxylase; polyamine;
D O I：
10.1016/S0304-3835(00)00511-5
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Resveratrol, a natural polyphenolic phytoalexine present in grapes and wines, has been reported to exert a variety of important pharmacological effects. We investigated the effects of resveratrol on the growth and polyamine metabolism of CaCo-2 human colon cancer cells. Treatment of the CaCo-2 cells with 25 muM resveratrol caused a 70% growth inhibition. The cells accumulated at the S/G2 phase transition of the cell cycle. No signs of cytotoxicity or apoptosis were detected. Resveratrol caused a significant decrease of ornithine decarboxylase (ODC) activity, a key enzyme of polyamine biosynthesis, which is enhanced in cancer growth. ODC inhibition resulted in the reduction of the intracellular putrescine content, indicating that polyamines might represent one of several targets involved in the anti-proliferative effects of resveratrol. (C) 2000 Elsevier Science ireland Ltd. All rights reserved.
引用
收藏
页码：85 / 91
页数：7
相关论文
共 36 条
[1]   POLYAMINE STARVATION PROLONGS THE S-PHASE AND G2-PHASE OF POLYAMINE-DEPENDENT (ARGINASE-DEFICIENT) CHO CELLS [J].
ANEHUS, S ;
POHJANPELTO, P ;
BALDETORP, B ;
LANGSTROM, E ;
HEBY, O .
MOLECULAR AND CELLULAR BIOLOGY, 1984, 4 (05) :915-922
[2]   Resveratrol inhibits metal ion-dependent and independent peroxidation of porcine low-density lipoproteins [J].
Belguendouz, L ;
Fremont, L ;
Linard, A .
BIOCHEMICAL PHARMACOLOGY, 1997, 53 (09) :1347-1355
[3]   Resveratrol, a natural product present in wine, decreases tumour growth in a rat tumour model [J].
Carbó, N ;
Costelli, P ;
Baccino, FM ;
López-Soriano, FJ ;
Argilés, JM .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1999, 254 (03) :739-743
[4]  
Clément MV, 1998, BLOOD, V92, P996
[5]  
COHEN SS, 1998, GUIDE POLYAMINES, P184
[6]   ORNITHINE DECARBOXYLASE AND DIAMINE OXIDASE IN HUMAN-COLON CARCINOMA CELL-LINE CACO-2 IN CULTURE [J].
DAGOSTINO, L ;
DANIELE, B ;
PIGNATA, S ;
GENTILE, R ;
TAGLIAFERRI, P ;
CONTEGIACOMO, A ;
SILVESTRO, G ;
POLISTINA, C ;
BIANCO, AR ;
MAZZACCA, G .
GASTROENTEROLOGY, 1989, 97 (04) :888-894
[7]   Resveratrol arrests the cell division cycle at S/G2 phase transition [J].
Della Ragione, F ;
Cucciolla, V ;
Borriello, A ;
Della Pietra, V ;
Racioppi, L ;
Soldati, G ;
Manna, C ;
Galletti, P ;
Zappia, V .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1998, 250 (01) :53-58
[8]   Concomitant changes in polyamine pools and DNA methylation during growth inhibition of human colonic cancer cells [J].
Duranton, B ;
Keith, G ;
Gossé, F ;
Bergmann, C ;
Schleiffer, R ;
Raul, F .
EXPERIMENTAL CELL RESEARCH, 1998, 243 (02) :319-325
[9]   Modulating effect of resveratrol and quercetin on oral cancer cell growth and proliferation [J].
ElAttar, TMA ;
Virji, AS .
ANTI-CANCER DRUGS, 1999, 10 (02) :187-193
[10]   Comparative study of radical scavenger and antioxidant properties of phenolic compounds from Vitis vinifera cell cultures using in vitro tests [J].
Fauconneau, B ;
WaffoTeguo, P ;
Huguet, F ;
Barrier, L ;
Decendit, A ;
Merillon, JM .
LIFE SCIENCES, 1997, 61 (21) :2103-2110
1234