Producing Acetic Acid of Acetobacter pasteurianus by Fermentation Characteristics and Metabolic Flux Analysis

被引：17
|
作者：
Wu, Xuefeng [1 ,2 ]
Yao, Hongli [1 ]
Liu, Qing [1 ]
Zheng, Zhi [1 ,2 ]
Cao, Lili [1 ,2 ]
Mu, Dongdong [1 ]
Wang, Hualin [1 ,2 ]
Jiang, Shaotong [1 ,2 ]
Li, Xingjiang [1 ,2 ]
机构：
[1] Hefei Univ Technol, Sch Food Sci & Engn, 193 Tunxi Rd, Hefei 230009, Anhui, Peoples R China
[2] Key Lab Agr Prod Proc Anhui Prov, Hefei 230009, Anhui, Peoples R China
来源：
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2018年 / 186卷 / 01期
基金：
中国国家自然科学基金;
关键词：
Acetic acid fermentation; Ethanol respiratory chain; Acetobacter pasteurianus [!text type='JS']JS[!/text]T-S; Environmental stress; Metabolic flux analysis; QUINOPROTEIN ALCOHOL-DEHYDROGENASE; VINEGAR FERMENTATION; HIGHER TEMPERATURES; ACETATE OXIDATION; BACTERIA; CHROMATOGRAPHY; ACETIFICATION; RESISTANCE; PATHWAYS; STRAIN;
D O I：
10.1007/s12010-018-2732-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The acetic acid bacterium Acetobacter pasteurianus plays an important role in acetic acid fermentation, which involves oxidation of ethanol to acetic acid through the ethanol respiratory chain under specific conditions. In order to obtain more suitable bacteria for the acetic acid industry, A. pasteurianus JST-S screened in this laboratory was compared with A. pasteurianus CICC 20001, a current industrial strain in China, to determine optimal fermentation parameters under different environmental stresses. The maximum total acid content of A. pasteurianus JST-S was 57.14 +/- 1.09 g/L, whereas that of A. pasteurianus CICC 20001 reached 48.24 +/- 1.15 g/L in a 15-L stir stank. Metabolic flux analysis was also performed to compare the reaction byproducts. Our findings revealed the potential value of the strain in improvement of industrial vinegar fermentation.
引用
收藏
页码：217 / 232
页数：16
相关论文
共 50 条
  • [41] Transcriptome response of Acetobacter pasteurianus Ab3 to high acetic acid stress during vinegar production
    Kai Xia
    Chengcheng Han
    Jun Xu
    Xinle Liang
    Applied Microbiology and Biotechnology, 2020, 104 : 10585 - 10599
  • [42] Effect of culture conditions on producing and uptake hydrogen flux of biohydrogen fermentation by metabolic flux analysis method
    Niu, Kun
    Zhang, Xu
    Tan, Wen-Song
    Zhu, Ming-Long
    BIORESOURCE TECHNOLOGY, 2011, 102 (15) : 7294 - 7300
  • [43] Investigation of lipid profile in Acetobacter pasteurianus Ab3 against acetic acid stress during vinegar production
    Chengcheng Han
    Kai Xia
    Jieqiong Yang
    Hong Zhang
    Matthew P. DeLisa
    Xinle Liang
    Extremophiles, 2020, 24 : 909 - 922
  • [44] Analysis of proteins responsive to acetic acid in Acetobacter:: Molecular mechanisms conferring acetic acid resistance in acetic acid bacteria
    Nakano, Shigeru
    Fukaya, Masahiro
    INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2008, 125 (01) : 54 - 59
  • [45] KINETIC STUDIES ON SUBMERGED ACETIC-ACID FERMENTATION .3. EFFICIENCY OF ENERGY METABOLISM IN ACETIC-ACID FERMENTATION USING ACETOBACTER-RANCENS
    MORI, A
    TERUI, G
    JOURNAL OF FERMENTATION TECHNOLOGY, 1972, 50 (08): : 510 - &
  • [46] Variation of Initial pH and Ethanol Concentration onAcetic Acid Fermentation by Immobilized Cell of Acetobacter pasteurianus INT-7
    Luwihana, Sri
    Kuswanto, Kapti Rahayu
    Rahayu, Endang Sutriswati
    Sudarmadji, Slamet
    AGRITECH, 2010, 30 (02): : 123 - 132
  • [47] Investigation of Acid Tolerance Mechanism of Acetobacter pasteurianus under Different Concentrations of Substrate Acetic Acid Based on 4D Label-Free Proteomic Analysis
    Li, Tian
    Wang, Xinwei
    Li, Chunyan
    Fu, Qingquan
    Shi, Xuewei
    Wang, Bin
    FOODS, 2023, 12 (24)
  • [48] Complete Genome Sequencing and Comparative Genomic Analysis of the Thermotolerant Acetic Acid Bacterium, Acetobacter pasteurianus SKU1108, Provide a New Insight into Thermotolerance
    Matsutani, Minenosuke
    Hirakawa, Hideki
    Hiraoka, Eri
    Theeragool, Gunjana
    Yakushi, Toshiharu
    Matsushita, Kazunobu
    MICROBES AND ENVIRONMENTS, 2016, 31 (04) : 395 - 400
  • [49] The low biomass yields of the acetic acid bacterium Acetobacter pasteurianus are due to a low stoichiometry of respiration-coupled proton translocation
    Luttik, MAH
    vanSpanning, R
    Schipper, D
    vanDijken, JP
    Pronk, JT
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (09) : 3345 - 3351
  • [50] Disruption of the groEL Gene Revealed A Physiological Role for Chaperonin in the Thermotolerant Acetic Acid Bacterium, Acetobacter pasteurianus SKU1108
    Theeragool, Gunjana
    Pitiwittayakul, Nittaya
    Matsutani, Minenosuke
    Matsushita, Kazunobu
    CHIANG MAI JOURNAL OF SCIENCE, 2018, 45 (04): : 1623 - 1633
12345