Molecular cloning of human hyaluronan synthase

被引：100

作者：

Itano, N ^{[1
]}

Kimata, K ^{[1
]}

机构：

[1] AICHI MED UNIV, INST MOLEC SCI MED, NAGAKUTE, AICHI 48011, JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1996年 / 222卷 / 03期

关键词：

D O I：

10.1006/bbrc.1996.0827

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report here the cDNA sequence of human hyaluronan synthase. The cDNA clone was isolated from a human fetal brain cDNA library by screening with a DNA probe generated from the coding sequence of murine hyaluronan synthase. It contains a 2107 bp cDNA insert which is composed of a 1629 bp open reading frame and a short segment of the 3'-untranslated region. Comparative analysis of the predicted coding region with the murine hyaluronan synthase sequence indicates 84.4% and 96.0% identity in nucleotide sequence and amino acid sequences, respectively. Northern analysis indicated that the gene was ubiquitously expressed in various human tissues. (C) 1996 Academic Press, Inc.

引用

页码：816 / 820

页数：5

共 12 条

[1] THE STREPTOCOCCUS-PYOGENES HYALURONAN SYNTHASE - SEQUENCE COMPARISON AND CONSERVATION AMONG VARIOUS GROUP A-STRAINS [J].

DEANGELIS, PL ;

YANG, N ;

WEIGEL, PH .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1994, 199 (01) :1-10

[2]

ENGSTROMLAURENT A, 1989, CIBA F SYMP, V143, P150

[3]

IOZZO RV, 1985, LAB INVEST, V53, P373

[4] Expression cloning and molecular characterization of HAS protein, a eukaryotic hyaluronan synthase [J].

Itano, N ;

Kimata, K .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (17) :9875-9878

[5]

KIMATA K, 1983, CANCER RES, V43, P1347

[6]

KNUDSON W, 1989, CIBA F SYMP, V143, P150

[7] AN ANALYSIS OF VERTEBRATE MESSENGER-RNA SEQUENCES - INTIMATIONS OF TRANSLATIONAL CONTROL [J].

KOZAK, M .

JOURNAL OF CELL BIOLOGY, 1991, 115 (04) :887-903

[8] A SIMPLE METHOD FOR DISPLAYING THE HYDROPATHIC CHARACTER OF A PROTEIN [J].

KYTE, J ;

DOOLITTLE, RF .

JOURNAL OF MOLECULAR BIOLOGY, 1982, 157 (01) :105-132

[9] HYALURONAN [J].

LAURENT, TC ;

FRASER, JRE .

FASEB JOURNAL, 1992, 6 (07) :2397-2404

[10] CHARACTERIZATION OF A HIGH-MR PLASMA-MEMBRANE-BOUND PROTEIN AND ASSESSMENT OF ITS ROLE AS A CONSTITUENT OF HYALURONATE SYNTHASE COMPLEX [J].

MIAN, N .

BIOCHEMICAL JOURNAL, 1986, 237 (02) :343-357

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