Regulation of a virus-induced lethal disease in tomato revealed by LongSAGE analysis

被引：17
作者：
Irian, Saeed [1 ]
Xu, Ping [1 ]
Dai, Xinbin [1 ]
Zhao, Patrick X. [1 ]
Roossinck, Marilyn J. [1 ]
机构：
[1] Samuel Roberts Noble Fdn Inc, Ardmore, OK 73401 USA
来源：
MOLECULAR PLANT-MICROBE INTERACTIONS | 2007年 / 20卷 / 12期
关键词：
programmed cell death;
D O I：
10.1094/MPMI-20-12-1477
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Infection of Cucumber mosaic virus (CMV) and D satellite RNA (satRNA) in tomato plants induces rapid plant death, which has caused catastrophic crop losses. We conducted long serial analysis of gene expression (LongSAGE) in control and virus-infected plants to identify the genes that may be involved in the development of this lethal tomato disease. The transcriptomes were compared between mockinoculated plants and plants infected with CMV, CMV/D satRNA, or CMV/Dm satRNA (a nonnecrogenic mutant of D satRNA with three mutated nucleotides). The analysis revealed both general and specific changes in the tomato transcriptome after infection with these viruses. A massive transcriptional difference of approximately 400 genes was found between the transcriptornes of CMV/D and CMV/Dm satRNA-infected plants. Particularly, the LongSAGE data indicated the activation of ethylene synthesis and signaling by CMV/D satRNA infection. Results from inoculation tests with an ethylene-insensitive mutant and treatments with an ethylene action inhibitor further confirmed the role of ethylene in mediating the epinastic leaf symptoms and the secondary cell death in the stem. Results from Northern blot analysis demonstrated the partial contribution of ethylene in the induced defense responses in CMV/D satRNA-infected plants.
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收藏
页码：1477 / 1488
页数：12
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