Optimized method for sensitive detection of Shigatoxin-producing E-coli (STEC) in vegetarian foods

被引：0
|
作者：
Gallien, P [1 ]
Timm, M [1 ]
Lehmann, S [1 ]
Brandt, U [1 ]
Schiller, A [1 ]
机构：
[1] Natl Vet Med Referenzlabor E Coli, Bundesinst Risikoberwertung, D-06846 Dessau, Germany
来源：
DEUTSCHE LEBENSMITTEL-RUNDSCHAU | 2005年 / 101卷 / 05期
关键词：
STEC; EHEC; vegetarian foods; apple juice; lettuce; ELISA; PCR; VT-colony-immunoblot; Shigatoxin;
D O I：
暂无
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
An optimized method for sensitive detection of Shigatoxin-producing E coli (STEC) in vegetarian foods (apple juice and lettuce) is given. The method is independent from serotype of STEC. Furthermore this procedure is different from those used for investigation of foods originated from animals ( 35 LMBG (L07,18-1) und DIN 10118). One step cultivation for 18 h at +37 degrees C is necessary. The enriched material is used for making a screening test. ELISA and PCR were brought in. When using PCR a modification step for sample preparation is necessary. In case of a Shigatoxin (Stx) or Shigatoxin gene (stx) positive result the specific STEC isolation has to be done by using a VT-colony-immunoblot. The detection limit is 50 cfu/25 g (ml) food. The sensitivity in case of investigation of sprouts from different origin is insufficient. The method describe above should not be used.
引用
收藏
页码：177 / 182
页数：6
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