Acute changes in free and extracellular vesicle-associated circulating miRNAs and myokine profile in professional sky-runners during the Gran Sasso d'Italia vertical run

被引：6

作者：

Faraldi, M. ^{[1
]}

Sansoni, V. ^{[1
]}

Perego, S. ^{[1
]}

Gomarasca, M. ^{[1
]}

Gerosa, L. ^{[2
]}

Ponzetti, M. ^{[3
]}

Rucci, N. ^{[3
]}

Banfi, G. ^{[1
,4
]}

Lombardi, G. ^{[1
,5
]}

机构：

[1] IRCCS Ist Ortoped Galeazzi, Lab Expt Biochem & Mol Biol, Milan, Italy

[2] Grp Osped San Donato Fdn, Milan, Italy

[3] Univ LAquila, Dept Biotechnol & Appl Clin Sci, LAquila, Italy

[4] Univ Vita Salute San Raffaele, Milan, Italy

[5] Poznan Univ Phys Educ, Dept Athlet Strength & Conditioning, Poznan, Poland

来源：

FRONTIERS IN MOLECULAR BIOSCIENCES | 2022年 / 9卷

关键词：

physical exercise; circulating extracellular vesicles; circulating microRNAs; myokines; miRNAs target prediction; SKELETAL-MUSCLE CELLS; NEUROTROPHIC FACTOR; ENDOCRINE ORGAN; EXERCISE; MICRORNA; PROTEIN; BRAIN; MATRIX; OSTEONECTIN; ACTIVATION;

D O I：

10.3389/fmolb.2022.915080

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The modification of gene expression profile, a first step in adaptation to exercise, leads to changes in the level of molecules associated with skeletal muscle activity and energy metabolism-such as myokines-as well as those involved in their transcriptional regulation, like microRNA. This study aimed to investigate the influence of strenuous exercise on circulating microRNAs and their possible association with myokine response. Pre-competition and post-competition plasma samples were collected from 14 male athletes participating in a vertical run (+1,000 m gain, 3,600 m length). Circulating total (t-miRNA) and extracellular vesicle-associated (EV-miRNA) miRNAs were extracted from the pooled plasma. Nanoparticle tracking analysis was performed to investigate pre- and post-competition EV concentration and size distribution. A panel of 179 miRNAs was assayed by qPCR and analyzed by Exiqon GenEx v6 normalized on the global mean. t-miRNA and EV-miRNAs whose level was & GE;5-fold up- or down-regulated were validated for each single subject. Target prediction on MirWalk v3.0, Gene-Ontology, and pathway enrichment analysis on Panther v17.0 were performed to define the potential biological role of the identified miRNAs. A panel of 14 myokines was assayed in each sample by a multiplex immunoassay. In whole plasma, five miRNAs were upregulated and two were downregulated; in the EV fraction, five miRNAs were upregulated and three were downregulated. Nanoparticle tracking analysis revealed a similar EV size distribution in pre- and post-competition samples and a decreased concentration in post-competition samples related to pre-competition samples. Gene-Ontology and pathway enrichment analysis revealed that the identified t-miRNAs and EV-miRNAs were potentially involved in metabolism regulation in response to exercise. Correlation between fold-change of the post-competition relative to pre-competition plasma level of both t-miRNAs and EV-miRNAs and myokines further confirmed these results. This study provides an example of a systemic response to acute endurance exercise, in which circulating miRNAs play a pivotal role.

引用

页数：15