Transplanting embryonic stem cells onto damaged human corneal endothelium

被引：14

作者：

Hanson, Charles ^{[1
]}

Arnarsson, Arsaell ^{[2
]}

Hardarson, Thorir ^{[3
]}

Lindgard, Ann ^{[4
]}

Daneshvarnaeini, Mandana ^{[4
]}

Ellerstrom, Catarina ^{[5
]}

Bruun, Anita ^{[6
]}

Stenevi, Ulf ^{[4
]}

机构：

[1] Sahlgrens Univ Hosp, Unit Reprod Med, S-41345 Gothenburg, Sweden

[2] Univ Akureyri, Neurosci Lab, IS-600 Akureyri, Iceland

[3] Carlanderska Hosp, Fertil Ctr Scandinavia, S-40229 Gothenburg, Sweden

[4] Gothenburg Univ, Dept Ophthalmol, S-43180 Molndal, Sweden

[5] Takara Bio Europe AB, Arvid Wallgrens Backe 20, S-41346 Gothenburg, Sweden

[6] Lund Univ Hosp, Dept Ophthalmol, S-22121 Lund, Sweden

来源：

WORLD JOURNAL OF STEM CELLS | 2017年 / 9卷 / 08期

关键词：

Embryonic stem cells; Cornea; Descemet's membrane; Endothelium; Immunohistochemistry;

D O I：

10.4252/wjsc.v9.i8.127

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

AIM To investigate whether human embryonic stem cells (hESCs) could be made to attach, grow and differentiate on a human Descemet's membrane (DM). METHODS Spontaneously differentiated hESCs were transferred onto a human corneal button with the endothelial layer removed using ocular sticks. The cells were cultured on a DM for up to 15 d. The genetically engineered hESC line expressed green fluorescent protein, which facilitated identification during the culture experiments, tissue preparation, and analysis. To detect any differentiation into human corneal endothelial-like cells, we analysed the transplanted cells by immunohistochemistry using specific antibodies. RESULTS We found transplanted cells form a single layer of cells with a hexagonal shape in the periphery of the DM. The majority of the cells were negative for octamer-binding transcription factor 4 but positive for paired box 6 protein, sodium potassium adenosine triphosphatase (NaKATPase), and Zona Occludens protein 1. In four of the 18 trials, the transplanted cells were found to express CK3, which indicates that the stem cells differentiated into corneal epithelial cells in these cases. CONCLUSION It is possible to get cells originating from hESCs to become established on a human DM, where they grow and differentiate into corneal endothelial-like cells in vitro.

引用

页码：127 / 132

页数：6

共 9 条

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