Determination of fluoroquinolones in chicken feces - A new liquid-liquid extraction method combined with LC-MS/MS

被引：58

作者：

Janusch, Franziska ^{[1
]}

Scherz, Gesine ^{[2
]}

Mohring, Siegrun A. I. ^{[1
]}

Hamscher, Gerd ^{[1
]}

机构：

[1] Univ Giessen, Inst Food Chem & Food Biotechnol, D-35392 Giessen, Germany

[2] Univ Vet Med Hannover, Inst Pharmacol Toxicol & Pharm, D-30559 Hannover, Germany

来源：

ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY | 2014年 / 38卷 / 03期

关键词：

Fluoroquinolones; LC-MS/MS; Liquid-liquid extraction; Feces; Chicken; TANDEM MASS-SPECTROMETRY; ANTIBACTERIAL AGENTS; VETERINARY ANTIBIOTICS; ASSISTED EXTRACTION; ANIMAL MANURE; ENROFLOXACIN; SOIL; CHROMATOGRAPHY; CIPROFLOXACIN; RESIDUES;

D O I：

10.1016/j.etap.2014.09.011

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The application of antibiotics including fluoroquinolones to farming animals is widespread and may lead to the development of antibiotic resistance and other environmental effects. To calculate environmental loads and for a proper risk assessment it is necessary to determine the antibiotic concentration in feces. Therefore, a new liquid-liquid extraction method combined with HPLC-MS/MS for the detection of marbofloxacin, ciprofloxacin, enrofloxacin and difloxacin in chicken feces was developed. Recoveries ranged from 51.0% to 83.5%. LOQs were between 0.10 and 1.09 mu g/kg. Feces of chickens treated with an enrofloxacin dosage of 10 mg/kg bodyweight revealed maximum enrofloxacin and ciprofloxacin concentrations of 61.3 and 18.8 mg/kg. Both antibiotics could be detected in feces up to two days after the last application in notable amounts (similar to 1 mg/kg). Thus, feces of recently medicated chickens should not be used as a fertilizer without any further processing. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：792 / 799

页数：8

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