Proteomic analysis of zygote and ookinete stages of the avian malaria parasite Plasmodium gallinaceum delineates the homologous proteomes of the lethal human malaria parasite Plasmodium falciparum
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作者:
Patra, Kailash P.
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Univ Calif San Diego, Sch Med, Dept Med,Div Infect Dis, George Palade Labs, La Jolla, CA 92093 USAUniv Calif San Diego, Sch Med, Dept Med,Div Infect Dis, George Palade Labs, La Jolla, CA 92093 USA
Patra, Kailash P.
[1
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Johnson, Jeff R.
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Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USAUniv Calif San Diego, Sch Med, Dept Med,Div Infect Dis, George Palade Labs, La Jolla, CA 92093 USA
Johnson, Jeff R.
[2
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Cantin, Greg T.
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Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USAUniv Calif San Diego, Sch Med, Dept Med,Div Infect Dis, George Palade Labs, La Jolla, CA 92093 USA
Cantin, Greg T.
[2
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Yates, John R., III
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Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USAUniv Calif San Diego, Sch Med, Dept Med,Div Infect Dis, George Palade Labs, La Jolla, CA 92093 USA
Yates, John R., III
[2
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Vinetz, Joseph M.
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Univ Calif San Diego, Sch Med, Dept Med,Div Infect Dis, George Palade Labs, La Jolla, CA 92093 USAUniv Calif San Diego, Sch Med, Dept Med,Div Infect Dis, George Palade Labs, La Jolla, CA 92093 USA
Vinetz, Joseph M.
[1
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机构:
[1] Univ Calif San Diego, Sch Med, Dept Med,Div Infect Dis, George Palade Labs, La Jolla, CA 92093 USA
[2] Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA
Delineation of the complement of proteins comprising the zygote and ookinete, the early developmental stages of Plasmodium within the mosquito midgut, is fundamental to understand initial molecular parasite-vector interactions. The published proteome of Plasmodium falciparum does not include analysis of the zygote/ookinete stages, nor does that of P. berghei include the zygote stage or secreted proteins. P. gallinaceum zygote, ookinete, and ookinete-secreted/released protein samples were prepared and subjected to Multidimensional protein identification technology (MudPIT). Peptides of P. gallinaceum zygote, ookinete, and ookinete-secreted proteins were identified by MS/MS, mapped to ORFs (>50 amino acids) in the extent P. gallinaceum whole genome sequence, and then matched to homologous ORFs in P. falciparum. A total of 966 P. falciparum ORFs encoding orthologous proteins were identified; just over 40% of these predicted proteins were found to be hypothetical. A majority of putative proteins with predicted secretory signal peptides or transmembrane domains were hypothetical proteins. This analysis provides a more comprehensive view of the hitherto unknown proteome of the early mosquito midgut stages of P. falciparum. The results underpin more robust study of Plasmodium-mosquito midgut interactions, fundamental to the development of novel strategies of blocking malaria transmission.
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Univ Stellenbosch, Dept Biochem, Private Bag X1, ZA-7602 Matieland, South AfricaUniv Stellenbosch, Dept Biochem, Private Bag X1, ZA-7602 Matieland, South Africa
van Niekerk, David D.
Penkler, Gerald P.
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Univ Stellenbosch, Dept Biochem, Private Bag X1, ZA-7602 Matieland, South Africa
Vrije Univ Amsterdam, Mol Cell Physiol, Amsterdam, NetherlandsUniv Stellenbosch, Dept Biochem, Private Bag X1, ZA-7602 Matieland, South Africa
Penkler, Gerald P.
du Toit, Francois
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Univ Stellenbosch, Dept Biochem, Private Bag X1, ZA-7602 Matieland, South AfricaUniv Stellenbosch, Dept Biochem, Private Bag X1, ZA-7602 Matieland, South Africa
du Toit, Francois
Snoep, Jacky L.
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Univ Stellenbosch, Dept Biochem, Private Bag X1, ZA-7602 Matieland, South Africa
Vrije Univ Amsterdam, Mol Cell Physiol, Amsterdam, Netherlands
Univ Manchester, MIB, Manchester M13 9PL, Lancs, EnglandUniv Stellenbosch, Dept Biochem, Private Bag X1, ZA-7602 Matieland, South Africa
机构:
Natl Inst Malaria Res, Indian Council Med Res, Parasite Host Biol Grp, Sect 8, New Delhi 110077, IndiaNatl Inst Malaria Res, Indian Council Med Res, Parasite Host Biol Grp, Sect 8, New Delhi 110077, India
Tiwari, Savitri
Sharma, Nivedita
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Natl Inst Malaria Res, Indian Council Med Res, Parasite Host Biol Grp, Sect 8, New Delhi 110077, IndiaNatl Inst Malaria Res, Indian Council Med Res, Parasite Host Biol Grp, Sect 8, New Delhi 110077, India
Sharma, Nivedita
Sharma, Guru Prasad
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Versiti Blood Ctr Wisconsin, Milwaukee, WI 53233 USANatl Inst Malaria Res, Indian Council Med Res, Parasite Host Biol Grp, Sect 8, New Delhi 110077, India
Sharma, Guru Prasad
Mishra, Neelima
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Natl Inst Malaria Res, Indian Council Med Res, Parasite Host Biol Grp, Sect 8, New Delhi 110077, IndiaNatl Inst Malaria Res, Indian Council Med Res, Parasite Host Biol Grp, Sect 8, New Delhi 110077, India
机构:
Columbia Univ, Irving Med Ctr, Dept Microbiol & Immunol, New York, NY 10027 USAColumbia Univ, Irving Med Ctr, Dept Microbiol & Immunol, New York, NY 10027 USA
Anton, Leonie
Cobb, David W.
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Columbia Univ, Irving Med Ctr, Dept Microbiol & Immunol, New York, NY 10027 USAColumbia Univ, Irving Med Ctr, Dept Microbiol & Immunol, New York, NY 10027 USA
Cobb, David W.
Ho, Chi-Min
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Columbia Univ, Irving Med Ctr, Dept Microbiol & Immunol, New York, NY 10027 USAColumbia Univ, Irving Med Ctr, Dept Microbiol & Immunol, New York, NY 10027 USA