Proteomic analysis of zygote and ookinete stages of the avian malaria parasite Plasmodium gallinaceum delineates the homologous proteomes of the lethal human malaria parasite Plasmodium falciparum

被引:31
|
作者
Patra, Kailash P. [1 ]
Johnson, Jeff R. [2 ]
Cantin, Greg T. [2 ]
Yates, John R., III [2 ]
Vinetz, Joseph M. [1 ]
机构
[1] Univ Calif San Diego, Sch Med, Dept Med,Div Infect Dis, George Palade Labs, La Jolla, CA 92093 USA
[2] Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA
关键词
malaria; MudPIT; ookinete; zygote;
D O I
10.1002/pmic.200700727
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Delineation of the complement of proteins comprising the zygote and ookinete, the early developmental stages of Plasmodium within the mosquito midgut, is fundamental to understand initial molecular parasite-vector interactions. The published proteome of Plasmodium falciparum does not include analysis of the zygote/ookinete stages, nor does that of P. berghei include the zygote stage or secreted proteins. P. gallinaceum zygote, ookinete, and ookinete-secreted/released protein samples were prepared and subjected to Multidimensional protein identification technology (MudPIT). Peptides of P. gallinaceum zygote, ookinete, and ookinete-secreted proteins were identified by MS/MS, mapped to ORFs (>50 amino acids) in the extent P. gallinaceum whole genome sequence, and then matched to homologous ORFs in P. falciparum. A total of 966 P. falciparum ORFs encoding orthologous proteins were identified; just over 40% of these predicted proteins were found to be hypothetical. A majority of putative proteins with predicted secretory signal peptides or transmembrane domains were hypothetical proteins. This analysis provides a more comprehensive view of the hitherto unknown proteome of the early mosquito midgut stages of P. falciparum. The results underpin more robust study of Plasmodium-mosquito midgut interactions, fundamental to the development of novel strategies of blocking malaria transmission.
引用
收藏
页码:2492 / 2499
页数:8
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