Insulin Tolerance Test under Anaesthesia to Measure Tissue-specific Insulin-stimulated Glucose Disposal

被引：8
|
作者：
Fazakerley, Daniel J. [1 ]
Fritzen, Andreas M. [1 ,2 ]
Nelson, Marin E. [1 ]
Thorius, Ida H. [1 ,2 ]
Cooke, Kristen C. [1 ]
Humphrey, Sean J. [1 ]
Cooney, Gregory J. [3 ]
James, David E. [1 ,3 ]
机构：
[1] Univ Sydney, Sch Life & Environm Sci, Charles Perkins Ctr, Camperdown, NSW 2006, Australia
[2] Univ Copenhagen, Fac Sci, Dept Nutr Exercise & Sports, Sect Mol Physiol, DK-2200 Copenhagen, Denmark
[3] Univ Sydney, Sydney Med Sch, Charles Perkins Ctr, Camperdown, NSW 2006, Australia
来源：
BIO-PROTOCOL | 2019年 / 9卷 / 02期
基金：
英国医学研究理事会;
关键词：
Insulin; Adipose tissue; Muscle; Glucose transport; Glucose uptake; Insulin tolerance test; IN-VIVO; ADIPOSE-TISSUE; MUSCLE; SENSITIVITY; METABOLISM; RESISTANCE; INVIVO;
D O I：
10.21769/BioProtoc.3146
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Insulin resistance is a pathophysiological state defined by impaired responses to insulin and is a risk factor for several metabolic diseases, most notably type 2 diabetes. Insulin resistance occurs in insulin target tissues including liver, adipose and skeletal muscle. Methods such as insulin tolerance tests and hyperinsulinaemic-euglycaemic clamps permit assessment of insulin responses in specific tissues and allow the study of the progression and causes of insulin resistance. Here we detail a protocol for assessing insulin action in adipose and muscle tissues in anesthetized mice administered with insulin intravenously.
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页数：13
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