Isolation and enrichment of mouse splenic T cells for ex vivo and in vivo T cell receptor stimulation assays

被引：18

作者：

Grosjean, Clemence ^{[1
]}

Quessada, Julie ^{[1
]}

Nozais, Mathis ^{[1
]}

Loosveld, Marie ^{[1
,2
]}

Payet-Bornet, Dominique ^{[1
]}

Mionnet, Cyrille ^{[1
]}

机构：

[1] Aix Marseille Univ, Ctr Immunol Marseille Luminy CIML, CNRS, INSERM,Case 906,, Parc Sci Luminy, F-13288 Marseille 9, France

[2] Hosp Timone, APHM, Lab Dhematol, Marseille, France

来源：

STAR PROTOCOLS | 2021年 / 2卷 / 04期

关键词：

Antibody; Cell Biology; Cell culture; Cell isolation; Cell-based Assays; Flow Cytometry/Mass Cytometry; Immunology; Model Organisms;

D O I：

10.1016/j.xpro.2021.100961

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Specific antigen recognition by T cell receptor (TCR) activates TCR signaling pathway, leading to T cell proliferation and differentiation into effector and memory cells. Herein, we describe protocols for TCR stimulation assays, including procedures for the isolation and enrichment of mouse splenic T cells for ex vivo TCR stimulation with anti-CD3/CD28 antibodies, and the use of ovalbumin-OT-II mouse model for in vivo TCR stimulation. We applied this protocol to show that MYC protein is essential for T cell proliferation and differentiation.For complete details on the use and execution of this protocol, please refer to Nozais et al. (2021).

引用

页数：15

共 8 条

[1] Defective TCR expression in transgenic mice constructed using cDNA-based α- and β-chain genes under the control of heterologous regulatory elements [J].