Chemical transformations in individual ultrasmall biomimetic containers

被引：225

作者：

Chiu, DT

Wilson, CF

Ryttsén, F

Strömberg, A

Farre, C

Karlsson, A

Nordholm, S

Gaggar, A

Modi, BP

Moscho, A

Garza-López, RA

Orwar, O

Zare, RN ^{[1
]}

机构：

[1] Stanford Univ, Dept Chem, Stanford, CA 94305 USA

[2] Univ Gothenburg, Dept Chem, S-41296 Gothenburg, Sweden

[3] Pomona Coll, Dept Chem, Claremont, CA 91711 USA

来源：

SCIENCE | 1999年 / 283卷 / 5409期

关键词：

D O I：

10.1126/science.283.5409.1892

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Individual phospholipid vesicles, 1 to 5 micrometers in diameter, containing a single reagent or a complete reaction system, were immobilized with an infrared laser optical trap or by adhesion to modified borosilicate glass surfaces. Chemical transformations were initiated either by electroporation or by electrofusion, in each case through application of a short (10-microsecond), intense (20 to 50 kilovolts per centimeter) electric pulse delivered across ultramicroelectrodes. Product formation was monitored by far-field laser fluorescence microscopy. The ultrasmall characteristic of this reaction volume led to rapid diffusional mixing that permits the study of fast chemical kinetics. This technique is also well suited for the study of reaction dynamics of biological molecules within lipid-enclosed nanoenvironments that mimic cell membranes.

引用

页码：1892 / 1895

页数：4

共 24 条

[1] CHANGES IN MEMBRANE-STRUCTURE INDUCED BY ELECTROPORATION AS REVEALED BY RAPID-FREEZING ELECTRON-MICROSCOPY [J].