Up-regulation of inhibitors of DNA binding/differentiation gene during alendronate-induced osteoblast differentiation

被引：15

作者：

Kang, Ae Ra ^{[2
]}

Oh, Young Rim ^{[2
]}

Kim, Heung Yeol ^{[2
]}

Park, Min Jung ^{[3
]}

Joo, Bo Sun ^{[3
]}

Choi, Won Jun ^{[4
]}

Lee, Ji Young ^{[5
]}

Jung, Min Hyung ^{[6
]}

Ji, Yong Il ^{[7
]}

Choi, Jong Soon ^{[1
]}

机构：

[1] Kosin Univ, Coll Med, Dept Family Med, Pusan, South Korea

[2] Kosin Univ, Coll Med, Dept Obstet & Gynecol, Pusan, South Korea

[3] Good Moonhwa Hosp, Ctr Reprod Med & Infertil, Pusan, South Korea

[4] Gyeongsang Natl Univ, Sch Med, Dept Obstet & Gynecol, Jinju, South Korea

[5] Konkuk Univ, Dept Obstet & Gynecol, Sch Med, Seoul, South Korea

[6] Kyung Hee Univ, Sch Med, Dept Obstet & Gynecol, Seoul, South Korea

[7] Inje Univ, Dept Obstet & Gynecol, Sch Med, Haeundaepaik Hosp, Pusan, South Korea

来源：

ARCHIVES OF GYNECOLOGY AND OBSTETRICS | 2012年 / 285卷 / 05期

关键词：

Alendronate; Osteoblasts; Osteoporosis; OSTEOGENIC DIFFERENTIATION; BONE; BISPHOSPHONATES; PROLIFERATION; PROTEINS; BINDING; PATHWAY;

D O I：

10.1007/s00404-011-2141-1

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

To investigate the effect of alendronate on the expression of Id genes in osteoblast differentiation. C2C12 cells were treated with alendronate for various concentrations and time periods. For evaluation of alendronate-induced osteoblast differentiation in C2C12 cells, alkaline phosphatase (ALP) activity was measured. The expression of osteoblast differentiation markers such as ALP, type-1 collagen (Col 1), and osteocalcin (OCN), and the expression of Id-1 and Id-2 were measured by RT-PCR. In order to understand the mechanism underlying the regulation of Id genes, the promoter region of the Id-1 gene was identified. Database analysis of the promoter region for Id-1 using known consensus sequences identified several putative response elements, including CCAAT/enhancer-binding protein beta (C/EBP beta). Alendronate treatment significantly increased not only ALP activity but also the expression of ALP, Col 1, and OCN, Id-1 and Id-2. C/EBP beta and alendronate cooperatively increased the promoter activity and expression of Id-1. These results suggest that C/EBP beta-mediated Id-1 transcriptional activation may regulate alendronate-induced osteoblast differentiation of C2C12 cells.

引用

页码：1331 / 1338

页数：8

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