14-3-3σ regulation by p53 mediates a chemotherapy response to 5-fluorouracil in MCF-7 breast cancer cells via Akt inactivation

被引：18
作者：
Zheng, Guopei [4 ]
Xiong, Yan [3 ]
Yi, Sisi [4 ]
Zhang, Weijia [4 ]
Peng, Bo [4 ]
Zhang, Qiong [1 ,2 ]
He, Zhimin [1 ,2 ,4 ]
机构：
[1] Guangzhou Med Univ, Canc Res Inst, Guangzhou 510095, Guangdong, Peoples R China
[2] Guangzhou Med Univ, Canc Hosp, Guangzhou 510095, Guangdong, Peoples R China
[3] Guangzhou Med Univ, Dept Pharmacol, Guangzhou 510095, Guangdong, Peoples R China
[4] Cent S Univ, Xiangya Sch Med, Canc Res Inst, Changsha 410078, Hunan, Peoples R China
来源：
FEBS LETTERS | 2012年 / 586卷 / 02期
基金：
中国国家自然科学基金;
关键词：
Breast cancer; Drug resistance; Methylation; 14-3-3; sigma; Akt; p53; SUPPRESSES TUMOR-GROWTH; FACTOR-KAPPA-B; DNA-DAMAGE; PROGRESSION; EXPRESSION; RESISTANCE; APOPTOSIS; PLAYS;
D O I：
10.1016/j.febslet.2011.11.034
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We previously demonstrated that 14-3-3 sigma was downregulated in 5-fluorouracil (5-Fu)-resistant MCF-7 breast cancer cells (MCF-7/5-Fu). Here, we found that stably enhanced 14-3-3 sigma expression strengthened the effects of 5-Fu, Mitoxantrone and cDDP. 14-3-3 sigma stabilised the p53 protein and bound Akt to inhibit its activity and its downstream targets: survivin, Bcl-2 and NF-kappa B-p50. In addition, decreased p53 expression, but not promoter hypermethylation, was responsible for the downregulation of 14-3-3 sigma in MCF-7/5-Fu cells. Meanwhile, initial treatments with high concentrations of 5-Fu clearly induced 14-3-3 sigma and p53 expression in a time-dependent manner. 14-3-3 sigma-mediated molecular events that synergise with p53 may play important roles in the chemotherapy of breast cancer. (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：163 / 168
页数：6
相关论文
共 50 条
  • [41] Anticancer effects of liriodenine on the cell growth and apoptosis of human breast cancer MCF-7 cells through the upregulation of p53 expression
    Li, Zhi-Hua
    Gao, Jin
    Hu, Ping-Hua
    Xiong, Jian-Ping
    ONCOLOGY LETTERS, 2017, 14 (02) : 1979 - 1984
  • [42] miR-338-3p confers 5-fluorouracil resistance in p53 mutant colon cancer cells by targeting the mammalian target of rapamycin
    Han, Jia
    Li, Jie
    Tang, Kaijie
    Zhang, Huahua
    Guo, Bo
    Hou, Ni
    Huang, Chen
    EXPERIMENTAL CELL RESEARCH, 2017, 360 (02) : 328 - 336
  • [43] Icariin induces apoptosis in breast cancer MCF-7 cells by regulating the MELK mediated PI3K/AKT signaling pathway
    Qian, Danping
    Zhu, Yin
    Xu, Le
    Dong, Tingting
    Chen, Ting
    Yu, Zhenghong
    EUROPEAN JOURNAL OF GYNAECOLOGICAL ONCOLOGY, 2021, 42 (05) : 957 - 965
  • [44] Matrine reversed multidrug resistance of breast cancer MCF-7/ADR cells through PI3K/AKT signaling pathway
    Zhou, Bing-Gang
    Wei, Chang-Sheng
    Zhang, Song
    Zhang, Zhi
    Gao, Huan-min
    JOURNAL OF CELLULAR BIOCHEMISTRY, 2018, 119 (05) : 3885 - 3891
  • [45] The biological response of MCF7 breast cancer cells to proteosome inhibition or γ-radiation is unrelated to the level of p53 induction
    L. H. Fallis
    E. Richards
    D. J. O'Connor
    S. Zhong
    J. K. Hsieh
    G. Packham
    X. Lu
    Apoptosis, 1999, 4 : 99 - 107
  • [46] The biological response of MCF7 breast cancer cells to proteosome inhibition or γ-radiation is unrelated to the level of p53 induction
    Fallis, LH
    Richards, E
    O'Connor, DJ
    Zhong, S
    Hsieh, JK
    Packham, G
    Lu, X
    APOPTOSIS, 1999, 4 (02) : 99 - 107
  • [47] Calycosin and Genistein Induce Apoptosis by Inactivation of HOTAIR/p-Akt Signaling Pathway in Human Breast Cancer MCF-7 Cells
    Chen, Jian
    Lin, Changrong
    Yong, Wang
    Ye, Yu
    Huang, Zhaoquan
    CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 2015, 35 (02) : 722 - 728
  • [48] Paired-related homeobox 1 overexpression promotes multidrug resistance via PTEN/PI3K/AKT signaling in MCF-7 breast cancer cells
    Luo, Haoyue
    Cong, Shaobo
    Dong, Jiaojiao
    Jin, Litao
    Jiang, Dandan
    Wang, Xingang
    Chen, Qingfeng
    Li, Funian
    MOLECULAR MEDICINE REPORTS, 2020, 22 (04) : 3183 - 3190
  • [49] Model-based investigation of the transcriptional activity of p53 and its feedback loop regulation via 14-3-3σ
    Vera, J.
    Nikolov, S.
    Lai, X.
    Singh, A.
    Wolkenhauer, O.
    IET SYSTEMS BIOLOGY, 2011, 5 (05) : 293 - U72
  • [50] miR-205-3p promotes proliferation and reduces apoptosis of breast cancer MCF-7 cells and is associated with poor prognosis of breast cancer patients
    Qiu, Changhong
    Huang, Fei
    Zhang, Qing
    Chen, Wei
    Zhang, Huiting
    JOURNAL OF CLINICAL LABORATORY ANALYSIS, 2019, 33 (08)
12345