Electrochemistry of nano-scale bacterial surface protein layers on gold

被引:19
作者
Handrea, M
Sahre, M
Neubauer, A
Sleytr, UB
Kautek, W
机构
[1] Fed Inst Mat Res & Testing, Lab Thin Film Technol, D-12205 Berlin, Germany
[2] Ludwig Boltzmann Inst Mol Nanotechnol, Ctr Ultrastruct Res, A-1180 Vienna, Austria
关键词
S-layers crystallization; gold; electrochemical quartz microbalance; scanning force microscopy; XPS;
D O I
10.1016/S1567-5394(03)00047-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The mechanism of the recrystallization of nano-scale bacterial surface protein layers (S-layers) on solid substrates is of fundamental interest in the understanding and engineering of biomembranes and e.g. biosensors. In this context, the influence of the charging state of the substrate had to be clarified. Therefore, the electrochemical behaviour of the S-layers on gold electrodes has been investigated by in-situ electrochemical quartz microbalance (EQMB) measurements, scanning force microscopy (SFM) and small-spot X-ray photoelectron spectroscopy (SS-XPS) of potentiostatically emersed substrates. It was shown that the negatively charged bonding sites of the S-layer units (e.g. carboxylates) can bond with positively charged An surface atoms in the positively charged electrochemical double layer region positive of the point of zero charge (similar to - 0.8 V vs. saturated mercury-mercurous sulphate electrode). Surface conditions in other potential regions decelerated the recrystallization and fixation of S-layers. Time-resolved in-situ and ex-situ measurements demonstrated that two-dimensional S-layer crystal formation on gold electrodes can occur within few minutes in contrast to hours common in self-assembled monolayer (SAM) generation. These results proved that the recrystallization and fixation of 2D-crystalline S-layers on an electronic conductor can be influenced and controlled by direct electrochemical manipulation. (C) 2003 Elsevier B.V. All rights reserved.
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页码:1 / 8
页数:8
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