A simple and inexpensive DNA purification for malignant hyperthermia PCR detection in porcine hair roots

被引：7

作者：

Bauerová, M

Bauer, M

Vasícek, D

机构：

[1] Constantine Philosopher Univ, Nura 94974, Slovakia

[2] BioVendor Ltd, Nitra 94901, Slovakia

[3] Res Inst Anim Prod, Nitra 94992, Slovakia

来源：

MEAT SCIENCE | 1999年 / 51卷 / 04期

关键词：

DNA purification; PCR; malignant hyperthermia;

D O I：

10.1016/S0309-1740(98)00127-2

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

We isolated DNA from porcine hair roots by silica matrix. Purified DNA is suitable for PCR analysis of malignant hyperthermia in the pig breeds. The using of hair roots as a source of DNA provides noninvasivity and it is also convenient for breeders. We compared amplification of DNA purified by silica matrix and Prep-A-gene kit. DNA purification by silica is as effective as Prep-A-gene purification and it is advantageous because of the low price. (C) 1999 Elsevier Science Ltd. All rights reserved.

引用

页码：325 / 327

页数：3

共 9 条

[1]

BOYLE JS, 1995, TRENDS GENET, V1, P8

[2] IDENTIFICATION OF A MUTATION IN PORCINE RYANODINE RECEPTOR ASSOCIATED WITH MALIGNANT HYPERTHERMIA [J].